同位素质谱仪测定小麦植株各器官δ^(15)N值的考察  

δ^(15)N Values of Wheat Plant Analysis by Istope Ratio Mass Spectrometry

作　　者：谷淑波[1] 宋雪皎[1] 王树芸[1] 高居荣[1] GU Shu-bo;SONG Xue-jiao;WANG Shu-yun;GAO Ju-rong(Key Laboratory of Crop Ecophysiology and Farming System,Ministry of Agriculture/College of Agronomy,Shandong Agrictdtral University,Tai＇an 271018,Shandong China)

机构地区：[1]农业部作物生理生态与耕作重点实验室山东农业大学农学院,山东泰安271018

出　　处：《分析测试技术与仪器》2018年第3期129-135,共7页Analysis and Testing Technology and Instruments

基　　金：公益性行业科研专项(No.201503130)资助

摘　　要：建立了15N标记的小麦植株各器官中δ^(15)N值的元素分析仪-同位素比质谱仪联用技术(EA-IRMS)的测定方法.对仪器的稳定性和线性进行了条件优化,标准气N_2的同位素比值的标准偏差为0.03‰,在同位素信号值1e^(-9)~1e^(-8)A范围内,总体线性为0.009‰/n A.称取不同量的小麦粉标准物质(OAS/Isotope)测定δ^(15)N值,进样量为4 mg时标准偏差最小,为最佳称样量.对硫酸铵标准物质(IAEA-N2-6)、小麦粉标准物质和^(15)N标记小麦植株样品各器官进行测定,δ^(15)N值的标准偏差均小于5%,测定精度和准确度较好.方法为研究氮素在小麦体内运转及利用效率提供了有效的技术手段.A method is established for the determination of the δ15N values of stable isotope 15N-labeled wheat plants by the element analyzer-isotope ratio mass spectrometic（EA-IRMS） technology. The stability and linearity of the EA-IRMS system were tested. The results showed that the standard deviation of stability was 0.03‰ of standard N2. When the intensity of isotopic signal ranged from 1e-9 to 1e-8A, the linearity of EA-IRMS system was 0.009‰/nA. 4 mg is the best sample quantity for standard wheat flour （OAS/Isotope） because the standard deviation of δ15N values was the smallest. The precision and accuracy of the results for the standard ammonium sulfate （IAEA-N2-6）, standard wheat flour and the 13N-labeled wheat samples were analyzed and all the standard deviations of δ15N values were less than 5%. The method is rapid and accuracy for the determination of the δ15N values of stable isotope 15N-labeled wheat samples, it provides effective technical ways for the study of nitrogen translocation and use efficiency of wheat plant.

关 键 词：元素分析仪-同位素比质谱仪联用技术 15N标记 小麦 δ^15N值 

分 类 号：S132[农业科学—农业基础科学]