葡萄霜霉病菌长期保存方法的研究  

Research on the Plasmopara viticola long-term preservation methods

作　　者：宋双[1] 姜彩鸽[1] 王国珍[1] 樊仲庆 张怡[1] SONG Shuang;JIANG Caige;WANG Guozhen;FAN Zhongqing;ZHANG Yi(Institute of Plant Protection,Ningxia Academy of Agricultural Science,Yinchuan 750002,China)

机构地区：[1]宁夏农林科学院植物保护研究所,宁夏银川750002

出　　处：《中外葡萄与葡萄酒》2018年第5期30-33,共4页Sino-Overseas Grapevine & Wine

基　　金：宁夏农林科学院全产业链创新示范项目(NKYZ-16-06-03);现代农业产业技术体系专项资金(CARS-30)

摘　　要：为了解决葡萄霜霉病菌的活体保存问题,本文开展了多种保存方法的研究。采用5%二甲基亚砜低温冷冻保存、液氮冷冻保存和琼脂粉包裹冷冻保存等三种方式,调查不同低温条件和不同保存时间对孢子囊致病性的影响。结果表明:用不同保护剂处理后葡萄霜霉病菌单斑病叶保存于-80℃中30 d,5%二甲基亚砜处理的孢子囊经回接后发病面积百分率为69.32%,其致病性明显高于液氮冷冻处理和琼脂粉包裹处理;低温保存条件下,-80℃中保存的孢子囊回接后致病性明显高于-20℃的处理;三种保存方式均表现随着保存时间的延长,致病性逐渐降低。In order to solve the problem of Plasmopara viticola preservation, pathogenicity of sporocysts under di？erent low temperature conditions and di？erent storage time for 5% dimethyl sulfoxide cryopreservation, liquid nitrogen cryopreservation and agar wrapped cryopreservation in？uences were studied. The results showed that the sporangia preserved at -80 ℃ for 30 days, and the virulent area of sporangia treated with 5% dimethyl sulfoxide cryopreservation was 69.32%, the infected was significantly higher than that of frozen in nitrogen liquid and agar refrigeration conditions; the sporangia preserved at -80 ℃ higher than those kept at -20 ℃. Three methods pathogenicity were decreased with the extension of time, the prolongation of the pathogenicity was gradually reduced.

关 键 词：葡萄霜霉病菌 保存剂 保存温度 保存时间 

分 类 号：Q93-336[生物学—微生物学]