不同浓度葡萄糖与脂多糖对SD大鼠胰腺β细胞胰岛素分泌的影响  

Influence of glucose and lipopolysaccharide on insulin secretion in pancreatic β cells of Sprague-Dawley rats

作　　者：裘年存 刘庆 杜植鹏 胡茂飞 高新春 王春喜 张伟[2] 单成祥[2] 仇明[2] 林惠 QIU Nian-cun;LIU Qing;DU Zhi-peng;Hu Mao-fei;Xin-chun;WANG Chun-xi;ZAHNG Wei;SHAN Cheng-xiang;QIU Ming;LIN Hui(Department of General Surgery,Hainan Branch of chinese Hospital（Sanya 572000,China;Department of General Surgery,Am ! to the Second Military Medical University（Shanghai 200003,China)

机构地区：[1]中国人民解放军总医院海南分院普通外科,海南三亚572000 [2]第二军医大学附属长征医院普外科,上海200003

出　　处：《中国现代普通外科进展》2018年第7期505-509,共5页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金(81370984;81270969;81300723)

摘　　要：目的:探索不同浓度葡萄糖(Glu)及脂多糖(LPS)对SD大鼠胰腺β细胞胰岛素分泌的影响。方法:体外原代培养8周龄大鼠胰腺组织,根据析因分析设计为4组:C+LPS 40(Glu 5 mmol/L+LPS 40pg/mL),C+LPS 200(Glu 5 mmol/L+LPS 200 pg/mL),HG+LPS 40(Glu 25 mmol/L+LPS 40 pg/mL),HG+LPS 200(Glu 25 mmol/L+LPS 200 pg/mL)。培养48 h后,利用实时荧光定量-聚合酶链反应(Q-PCR)检测这4组培养基内胰岛素m RNA表达,免疫荧光观察比较高糖和高LPS对大鼠胰腺内胰岛素合成情况的差异。结果:高糖和高LPS均能显著降低大鼠胰腺胰岛素的表达量(P<0.05)。高糖能使胰腺中胰岛素mRNA的表达下降39.58%(P<0.05),高LPS能使胰腺中胰岛素m RNA的表达下降40.50%(P<0.05),2种因素之间存在交互作用(P<0.05)。结论:高糖和高LPS均能对胰腺β细胞造成损伤,显著减少胰岛素的合成与释放。LPS对胰岛β细胞的损伤作用在T2DM的发生发展过程中起着重要作用。Objective： To investigate the effects of different concentrations of Glucose（Glu） and lipopolysaccharide （LPS） on insulin secretion in pancreatic β ceils of Sprague-Dawley（SD）rats. Methodsz According to the factorial analysis, the pancreatic tissues of 8-week old rats were cul- tured in vitro and randomized into 4 groups： C＋LPS40（Glu 5mmol/L＋ LPS 40 pg/mL）, C＋LPS 200（Glu 5 mmol/L＋LPS 200 pg/mL）, HG＋LPS40（GIu 25mmol/L＋LPS 40 pg/mL）, HG＋LPS 200（Glu 25 mmol/L＋LPS 200 pg/mL）. After 48 hours of culture, the expression of insulin mRNA in four groups were detected by Q-PCR. And immunoftuorescence was used to compare the differences between HG and LPS in insulin synthesis. Results： Both HG and LPS significantly reduce the expression of insulin In pan- creas（P〈0.05）. HG could decrease the expression of insulin mRNA by 39.58%（P〈0.05）.And high con- centration of LPS could decrease the expression of insulin mRNA by 40.50%（P〈0.05）. Besides, there existed interaction between the two factors（P〈0.05）. Conclusion： Both HG and LPS could damage the pancreatic [3 cells, and significantly reduce the synthesis and secretion of insulin. Therefore, the damage effect of LPS on the 15 cells in pancreatic islets plays an important role in the development and progression of Type 2 Diabetes Mellitus （T2DM）.

关 键 词：高糖 脂多糖 胰岛素 SD大鼠 

分 类 号：R587.1[医药卫生—内分泌]