燕麦肽缓解小鼠体力疲劳作用及机制  

Anti-fatigue effect of oat peptide in mice:an experimental study

作　　者：珠娜 毛瑞雪 刘睿[1] 乌兰 任金威[1] 李勇[1] ZHU Na;MAO Rui-xue;LIU Rui(Department of Nutrition and Food Hygiene,School of Public Health,Peking University Health Science Center,Beo＇ing 100191,China)

机构地区：[1]北京大学医学部公共卫生学院营养与食品卫生教研室,北京100191

出　　处：《中国公共卫生》2018年第9期1242-1245,共4页Chinese Journal of Public Health

摘　　要：目的探讨燕麦肽缓解体力疲劳作用及可能的作用机制。方法将192只SPF级雄性ICR小鼠随机分为6组:对照组、乳清蛋白组(0.22 g/kg)、4个燕麦肽组(0.50、1.00、2.00、4.00 g/kg),连续灌胃30 d;测定各组小鼠负重游泳力竭时间,采用吸光度法测定血乳酸含量,采用糖原试剂盒测定肝糖原含量和肌糖原含量,采用全自动生化分析仪测定血清尿素氮含量及乳酸脱氢酶活力。结果对照组与乳清蛋白组小鼠游泳时间分别为(3.90±1.38)和(4.91±2.21)min,差异无统计学意义(P>0.05);与乳清蛋白组比较,燕麦肽各剂量组小鼠负重游泳时间分别延长2.58、1.40、2.18、1.90倍,差异均有统计学意义(P<0.05)。对照组与乳清蛋白组小鼠血乳酸曲线下面积、肝糖原、肌糖原含量与乳酸脱氢酶活力均无明显差异;与乳清蛋白组比较,燕麦肽各剂量组小鼠血乳酸曲线下面积均明显降低,肝糖原含量均明显上升(P<0.05);与乳清蛋白组比较,燕麦肽2.00g/kg组小鼠肌糖原含量、乳酸脱氢酶活力升高(P<0.05)。结论燕麦肽具有一定程度缓解体力疲劳作用,其机制可能与减少血乳酸堆积,增加肝糖原含量有关。Objective To study whether the oat-peptide has anti-fatigue effect and possible mechanism of the effect.Methods A total of 192 male specific pathogen free（SPF） ICR mice were randomly divided into 6 groups： a control group,a whey protein（0.22 g/kg） group, and four oat-peptide groups（0.50, 1.00, 2.0, and 4.00 g/kg body weight）; all the mice were treated with intragastric administration for 30 days. Loading swimming time was determined; blood lactic acid was measured with absorption spectrophotometry; hepatic glycogen and muscle glycogen were detected with glycogen kit; and serum urea nitrogen and lactate dehydrogenase activity were determined with automatic biochemical analyzer. Results The mean loading swimming time of mice in the control and whey protein group was 3.90 ± 1.38 and 4.91 ± 2.21 minutes,and there was no significant difference in the time between the two groups（P〈0.05）. The loading swimming time of the mice in the four oat-peptide groups, compared with that in whey protein group, were prolonged significantly by 2.58, 1.40,2.18, and 1.90 times, respectively（P〈0.05 for all）. There were no significant differences between the control and whey protein group in the area under the blood lactate curve, the content of glycogen in liver and muscle, and the activity of lactate dehydrogenase. The under curve area of blood lactic acid of mice in all oat-peptide groups were significantly less than that in control group and whey protein group（both P〈0.05）. Compared with those in the whey protein group, the area of blood lactic acid curve in each of oat-peptide groups decreased significantly but the content of liver glycogen increased significantly（all P〈0.05）.Compared with those of the whey protein group, the muscle glycogen content and lactate dehydrogenase activity of 2.00 g/kg oat-peptide group increased. Conclusion Oat-peptide can relieve physical fatigue to some extent and its mechanism may relate to reducing the accumulation of blood lactic acid and increasing the conten

关 键 词：燕麦肽 抗疲劳 肝糖原 血乳酸 游泳时间 

分 类 号：R99[医药卫生—毒理学]