基于荧光铜纳米簇用于钴离子的超灵敏免标记检测  

Application of Fluorescent Copper Nanoclusters for Ultra Sensitive and Free Labeling Detection of Cobalt Ions

作　　者：王宁 爱军[1,2] WANG Ninga;AI Jun(College of Chemistry and Environmental Science;Key Laboratory of Physics and Chemistry of Functional Materials in the Inner Mongolia Autonomous Region,Inner Mongolia Normal University,Hohhot 010022,China)

机构地区：[1]内蒙古师范大学化学与环境科学学院,内蒙古呼和浩特010022 [2]内蒙古师范大学内蒙古自治区功能材料物理与化学重点实验室,内蒙古呼和浩特010022

出　　处：《化学试剂》2018年第9期859-862,共4页Chemical Reagents

基　　金：内蒙古自治区水环境安全协同创新培育中心项目(XTCX003);内蒙古自治区高等学校科学研究项目(NJZC16047);电分析化学国家重点实验室开放课题项目(SKLEAC201503)

摘　　要：为了检测浓度超标的重金属离子,探讨了一种新的荧光探针传感器检测法。利用柠檬酸钠作为还原剂合成铜纳米晶,然后加入半胱氨酸作为刻蚀剂和保护剂,与铜纳米晶配体交换反应合成荧光铜纳米簇,以铜纳米簇作为荧光探针建立了一种检测水样中重金属钴离子的方法。该方法是基于钴离子存在时,破坏铜纳米簇,导致荧光迅速降低。在较宽的钴离子浓度范围（1-100μmol/L）内,铜纳米簇荧光淬灭程度与浓度呈线性关系（R=0.970 0）,其检出限较低（0.567 1μmol/L）。铜纳米簇传感器特异性选择钴离子,不受其他金属离子干扰,检测的选择性较好。基于此,完成对钴离子的超灵敏免标记检测,且EDTA可作为钴离子的螯合剂,实现了铜纳米簇传感器的循环反复使用。A new fluorescent probe sensor was developed for the detection of heavy metal ions.The fluorescence of copper nanoclusters synthesized by using sodium citrate as reducing agent synthesis of nanocrystalline copper,then adding cysteine as etching agent and protective agent and copper nanocrystals ligand exchange reaction.A method for the detection of heavy metal cobalt ions in water samples was developed by using copper nanoclusters as fluorescent probes.The method is based on the presence of cobalt ions,which destroy the copper nanoclusters,resulting in the rapid reduction of fluorescence.When the concentration range of cobalt ions（1-100 μmol/L）,the fluorescence quenching degree of Cu nanoclusters showed a linear relationship（R=0.970 0）,and the detection limit was low（0.567 1 μmol/L）.The copper nanocluster sensor selects cobalt ions,which are not interfered by other metal ions,indicating that the selectivity of detection is good.Based on this,we complete the ultrasensitive label free detection of cobalt ions.And EDTA can be used as the chelating agent of cobalt ion,which can make use of the copper nanocluster sensor.

关 键 词：铜纳米簇 荧光检测 钴离子 

分 类 号：O652.3[理学—分析化学]