附子黑顺片多糖的提取及对免疫活性的研究  

Study on the extraction of polysaccharides from Heishunpian and its immune activity

作　　者：付业佩 杜宝香 范珊珊 李灿[1] 容蓉[1] 张颖颖[1] FU Ye-pei;DU Bao-xiang;FAN Shan-shan;LI Can;RONG Rong;ZHANG Ying-ying(Shandong University of Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,济南250355

出　　处：《中华中医药杂志》2018年第9期4147-4150,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81273626);国家科技重大专项项目(No.2014ZX09509001-001);山东省重点研发计划项目(No.2017CXGC1309);山东省重点产业关键技术创新工程项目(No.2016CYJS08A01-5);山东省高校中医药抗病毒协同创新中心项目(No.XTCX2014C01-02;No.XTCX2014B01-04)~~

摘　　要：目的:从附子黑顺片中提取粗多糖,并对其体内外免疫活性进行研究。方法:采用水提醇沉的方法提取黑顺片中粗多糖,苯酚-硫酸法测定其多糖含量,气相色谱法分析其单糖组成;分别用不同剂量的黑顺片多糖(200、100、50mg·kg^(-1)·d^(-1))灌胃由环磷酰胺所致的免疫低下模型小鼠,灌胃7d后检测脏器指数及一氧化氮(NO)、干扰素γ(IFN-γ)等指标;用MTT法研究黑顺片多糖对小鼠脾脏细胞、腹腔巨噬细胞的免疫作用影响。结果:黑顺片多糖含量为1.78%,其单糖组成主要是D-阿拉伯糖、D-木糖、D-葡萄糖和D-半乳糖;黑顺片多糖溶液均可促进脾脏和胸腺指数,高剂量组尤为明显(P<0.01);黑顺片多糖浓度为25μg/mL和25~100μg/mL时可分别促进小鼠脾脏细胞和腹腔巨噬细胞的增殖(P<0.05,P<0.01);高剂量组小鼠血清中NO和IFN-γ含量较模型组显著升高(P<0.05,P<0.01)。结论:黑顺片多糖对固有免疫及适应性免疫均有一定增强作用。Abstract： Objective： To extract polysaccharide from Heishunpian, and to primarily study on its immune activity. Methods： Polysaccharide was extracted from Heishunpian by water-extraction-alcohol-precipitation method, and determined by phenol sulfonic acid spectrophotometry. The monosaccharide composition was analyzed by gas chromatography. The mice were treated with different doses of Polysaccharide （200, 100, 50mg ·kg^-1. d^-1） for 7 days after intragastric administration of cyclophosphamide. The organ index, NO and IFN- 7 content were measured. The effects of polysaccharide on the immune function of mouse spleen cells and peritoneal macrophages were detected by MTT assay. Results： The content of polysaccharide of samples was 1.78%, and the polysaccharide was composed of D-arabinose, D-Xylose, D-glucose and D-galactose. Polysaccharide from Heishunpian could improve the spleen and thymus index, especially in high-dose group （P〈0.01）. The proliferation of spleen ceils and peritoneal macrophages in mice were promoted when the concentration of polysaccharides was 25μg/mL and 25-100μg/mL （P〈0.05, P〈0.01）. NO and IFN-γ level in serum from high-dose group increased significantly （P〈0.05, P〈0.01）. Conclusion： Polysaccharide from Heishunpian could significantly improve the innate and adoptive immunity in mice.

关 键 词：黑顺片多糖 多糖含量 单糖组成分析 细胞因子 环磷酰胺 体内外免疫活性 

分 类 号：R284[医药卫生—中药学]