清脑滴丸激活脑缺血再灌注大鼠海马JAK2/STAT3磷酸化的神经保护作用  

Neuroprotective effect of Qingnao Dripping Pills on activation of JAK2/STAT3 phosphorylation in hippocampus of rats with cerebral ischemia-reperfusion

作　　者：张昕洋 傅晨[2] 曾子修 陈宝鑫[2] 徐榛敏 陈志刚[3] 刘雪梅[2] ZHANG Xin-yangl;FU Chen;ZENG Zi-xiu;CHEN Bao-xin;XU Zhen-min;CHEN Zhi-gang;LIU Xue-mei(Beijing University of Chinese Medicine,Beijing 100029,China;Experimental Center,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Department of Neurology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院实验中心,北京100078 [3]北京中医药大学东方医院脑病一科,北京100078

出　　处：《中华中医药杂志》2018年第9期4184-4187,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81202683);国家重点基础研究发展计划(973计划)项目(No.2012CB518406);北京市自然科学基金项目(No.7182099)~~

摘　　要：目的:研究清脑滴丸对脑缺血再灌注大鼠海马Janus激酶2/信号转导子和转录激活子3(JAK2/STAT3)磷酸化蛋白(p-JAK2/p-STAT3)表达的影响。方法:采用线栓法制备大鼠大脑中动脉缺血(MCAO)再灌注模型,分为假手术组、MCAO组、清脑滴丸组。MCAO 1.5h再灌注24h处死大鼠,Garcia J H评分检测神经功能缺损程度,TTC染色检测脑梗死体积,Western blot法检测各组缺血侧海马JAK2/STAT3及其磷酸化蛋白p-JAK2/p-STAT3表达。结果:MCAO组脑梗死体积增大;与假手术组比较,MCAO组神经功能评分降低(P<0.05),p-JAK2/JAK2,p-STAT3/STAT3蛋白表达明显升高(P<0.01);与MCAO组比较,清脑滴丸组神经功能评分升高且脑梗死体积降低(P<0.05),p-JAK2/JAK2,p-STAT3/STAT3蛋白表达显著升高(P<0.01)。结论:清脑滴丸可能通过激活JAK2/STAT3磷酸化,发挥对脑缺血再灌注的神经保护作用。To study the effects of Qingnao Dripping Pills（QNDP） on the protein expression of Janus kinase 2/signal transducer and activator of transcription 3（JAK2/STAT3） phosphorylation（p-JAK2/p-STAT3） in hippocampus of rats with cerebral ischemia-reperfusion. Methods： The rat models of focal cerebral ischemia and reperfusion were made by thread occlusion technique induced middle cerebral artery occlusion. Rats were randomly divided into 3 groups： sham group, MCAO group and QNDP group. Rats were sacrificed after 1.5 h MCAO and 24 h reperfusion. Neurological deficits were evaluated by Garcia JH neurological score, cerebral infarct volume were measured by TTC staining. The protein expression of p-JAK2, JAK2, p-STAT3, STAT3 in ischemic hippocampus were detected by Western blot. Results： Cerebral infarction volume of the MCAO group was increased. Compared with the sham group, the neurological score of the MCAO group was decreased（P〈0.05）, the protein expression of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly increased in the MCAO group（P〈0.01）. Compared with the MCAO group, the neurological score of the QNDP group was increased and cerebral infarction volume was decreased（P〈0.05）, the protein expression of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly increased（P〈0.01）. Conclusion： The protective effects of QNDP against cerebral ischemia-reperfusion may be related to activate JAK2/STAT3 phosphorylation.

关 键 词：清脑滴丸 JAK2/STAT3信号通路 脑缺血再灌注 海马 

分 类 号：R285.5[医药卫生—中药学]