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作 者:王永慧[1,2] 陈建平[1] 高进[1] 张祥[2] 陈源[2] 陈德华[2] Yong-hui;CHEN Jian-ping;GAO Jin;ZHANG Xiang;CHEN Yuan;CHEN De-hua(Jiangsu Institute of Agricultural Sciences of Coastal Area/Ministry of Agriculture Observation and Experimental Station of Saline Land of Costal Area,Yancheng 224001,Jiangsu,China;jiangsu Province Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,Jiangsu,China).)
机构地区:[1]江苏沿海地区农业科学研究所/农业部沿海盐碱地农业科学观测实验站,江苏盐城224401 [2]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009
出 处:《应用生态学报》2018年第9期3017-3023,共7页Chinese Journal of Applied Ecology
基 金:国家自然科学基金项目(31501267);国家重点研发计划项目(2016YFD0101421);中国农业科学院科技创新工程项目(SYSFHT);江苏省三新工程项目[SXGC(2016)320];江苏省农业科技自主创新资金项目[CX(14)2065]资助~~
摘 要:为探讨土壤盐分对转Bt基因抗虫棉棉蕾抗虫性的影响程度,采用盆栽试验,以2个Bt棉品种‘新棉33B’(盐敏感)和‘中、07’(耐盐)为试验材料,设置5个土壤盐分水平(0、0.15%0.30%、0.45%和0.60%),研究盐分对蕾期Bt棉棉蕾中杀虫蛋白含量、Bt基因相对表达量及氮代谢相关酶和物质代谢的影响.结果表明:棉蕾中Bt杀虫蛋白含量随土壤盐分的升高而降低,与对照相比(0%),2个不同类型棉花品种在土壤盐分0.30%及以上时,棉蕾中杀虫蛋白含量显著下降,且随土壤盐分水平的升高,Bt杀虫蛋白含量下降幅度增大.土壤盐分胁迫提高了棉蕾中Bt基因的相对表达量.在相同土壤盐分水平下,盐敏感品种棉蕾中Bt杀虫蛋白含量受土壤盐分影响较大.杀虫蛋白表达量下降幅度大的品种,其可溶性蛋白含量、谷氨酸丙酮酸转氨酶(GPT)和谷氨酸草酰乙酸转氨酶(GOT)活性下降幅度较大,游离氨基酸含量、蛋白酶和肽酶活性上升幅度也较高.说明土壤盐分胁迫下,Bt棉棉蕾蛋白质合成能力下降,分解能力增强,可能是导致杀虫蛋白表达量下降的主要原因.To clarify the effects of soil salinity on the insect-resistance of flower buds in transgenic Btcotton of Xinmian 33 B(salt-sensitive) and Zhong 07(salt-tolerant), five levels(0, 0.15%,0.30%, 0.45% and 0.60%) of soil salinity were set to investigate the impacts of soil salinity on Bt protein content, relative expression ofBtgene, activities of nitrogen metabolism-related enzymes and substances in flower buds during flowering stage. The results showed that Bt protein contents of flower buds decreased with increasing soil salinity. The Bt protein contents of flower buds decreased significantly when the soil salinity level was above 0.30% in both cotton cutivars. Greater reduction of Bt protein content occurred under severe than moderate soil salinity stress. However, the relative expression ofCry1 Acgene increased in flower buds of both cultivars when the stress level increased.Greater decreases of Bt protein content of flower buds was observed in Xinmian 33 B compared to Zhong 07 under the same salinity level. The cultivars with greater reduction in Bt protein contents of flower buds also had greater reduction in the soluble protein content, glutamate pyruvate transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) activities, and greater increments in free amino acid contents, protease activities and peptidase activities. Therefore, the decrease of Bt protein content was caused by decreased synthesis and increased decomposition of protein in flower buds ofBtcotton under soil salinity stress.
关 键 词:土壤盐分 BT棉 杀虫蛋白 BT基因 棉蕾 氮代谢
分 类 号:S435.62[农业科学—农业昆虫与害虫防治]
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