富氢盐水对重度烧伤大鼠急性肾损伤的影响及机制  

Influences of hydrogen-rich saline on acute kidney injury in severely burned rats and mechanism

作　　者：王黎丽 郭松雪[3] 吴攀[1] 邵华伟[1] 韩春茂[1] Wang Lili;Guo Songxue;Wu Pan;Shao Huawei;Han Chunmao(Department of Burns,the Second Affilia-ted Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China)

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009 [2]解放军第九八医院烧伤整形科,313000 [3]浙江大学医学院附属第二医院整形科,杭州310009

出　　处：《中华烧伤杂志》2018年第9期629-636,共8页Chinese Journal of Burns

基　　金：国家自然科学基金面上项目（81671909）

摘　　要：目的探讨富氢盐水对大鼠重度烧伤后急性肾损伤的影响并分析相关机制。方法将56只SD大鼠按照随机数字表法分为假伤组8只、烧伤组24只、富氢盐水组24只。假伤组大鼠背部采用温水浴（20℃，15s）模拟致伤，烧伤组和富氢盐水组大鼠背部采用沸水浴法（100℃，15S）致Ⅲ度30％体表总面积的重度烫伤（以下称烧伤）。富氢盐水组大鼠伤后立即一次性腹腔注射富氢盐水10mL／kg，假伤组和烧伤组大鼠伤后立即注射同等剂量生理盐水。伤后6h3组大鼠均按4mL·kg-1·％TBSA-1腹腔注射乳酸钠林格液进行复苏。假伤组于伤后72h，烧伤组和富氢盐水组分别于伤后6、24、72h选取8只大鼠，取腹主动脉血后处死取肾脏组织，行苏木素一伊红染色观察组织病理学情况和进行肾小管损伤评分，临床血生化分析仪测定血肌酐和血尿素氮水平，免疫组织化学法观察肾脏组织中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL．1β）、IL-6的表达分布，实时荧光定量反转录PCR法检测肾脏组织中TNF-α、IL-1β、IL-6的mRNA表达，蛋白质印迹法检测高迁移率族蛋白B1（HMGB1）的蛋白表达。对数据行Kruskal-Wallis H检验、Dunn检验、单因素方差分析、Bonferroni检验。结果（1）假伤组大鼠伤后72h肾小管管腔结构完整，无炎性细胞浸润、细胞变形坏死。烧伤组大鼠自伤后6h肾小管逐步开始出现细胞空泡变形、管腔内碎裂蛋白聚集等损伤变化，并随着时间增加，损伤变化逐步加重。富氢盐水组大鼠肾脏组织伤后各时间点损伤情况较烧伤组同时间点有不同程度的减轻。与假伤组伤后72h比较，烧伤组、富氢盐水组大鼠伤后6、24、72h的肾小管损伤评分均明显升高（P〈0．05）；与烧伤组比较，富氢盐水组大鼠伤后6、24、72h的肾小管损伤评分均明显下降（P〈0．05）。（2）除富氢盐水组伤后6、72h（P〉0．05）Objective To explore the influences of hydrogen-rich saline on acute kidney injury in severely burned rats and to analyze the related mechanism. Methods Fifty-six Sprague Dawley rats were divided into sham injury group （ n = 8） , burn group （ n = 24） , and hydrogen-rich saline group （ n = 24） aceording to the random number table. Rats in sham injury group were treated by 20 ℃ water bath on the back for 15 s to simulate injury, and rats in burn group and hydrogen-rich saline group were inflicted with 30% total body surface area （TBSA） full-thickness scald （hereinafter referred to as burns） by 100℃ water bath on the back for 15 s. Immediately after injury, hydrogen-rich saline at the dose of 10 mL/kg were intra- peritoneally injected to the rats in hydrogen-rich saline group at one time, while normal saline with the same dose were intraperitoneally injected to the rats in sham injury group and burn group. At post injury hour （PIH） 6, rats in the 3 groups were intraperitoneally injected with 4 mL·kg-1.% TBSA -1 lactated Ringer＇ s solution for resuscitation. Eight rats from sham injury group at PIH 72 and eight rats from burn group and hydrogen-rich saline group at PIH 6, 24, and 72 were sacrificed respectively after their blood samples from abdominal aorta were collected. Then their kidney tissue was harvested for histopathologieal observation and renal tubular injury scoring by hematoxylin and eosin staining, serum creatinine and blood urea nitrogen were detected by the clinical blood biochemical analyzer, expression distribution and mRNA expressions of tumor necrosis factor α （TNF-α） , interleukin-1β （ IL-1β） , and IL-6 in renal tissue were evaluated by immunohis- tochemical staining and real time fluorescent quantitive reverse transcription polymerase chain reaction re- spectively, and protein expression of high mobility group protein 1 （HMGB1） was detected by Western blot- ting. Data were processed with Kruskal-Wallis H test, Dunn test, one-way analysis of variance,

关 键 词：烧伤 肾 氢 高迁移率族蛋白质类 炎症 

分 类 号：R644[医药卫生—外科学]