基于受激辐射信号的谱域光学相干层析分子成像方法  

Stimulated-emission based spectral domain optical coherence tomography for molecular contrast imaging

作　　者：胡喆皓 上官紫微 邱建榕 杨珊珊[1] 鲍文[1] 沈毅[1] 李鹏[1] 丁志华[1] Hu Zhe-Hao;Shangguan Zi-Wei;Qiu Jian-Rong;Fang Shan-Shan;Bao Wen;Shen Yi;Li Peng;Ding Zhi-Hua(College of Optical Science and Engineering,State Key Laboratory of Modern Optical Instrumentation,Zhejiang University,Hangzhou 310027,China)

机构地区：[1]浙江大学光电科学与工程学院现代光学仪器国家重点实验室,杭州310027

出　　处：《物理学报》2018年第17期99-107,共9页Acta Physica Sinica

基　　金：国家重点研发计划(批准号:2017YFA0700501);国家自然科学基金(批准号:61335003;61327007;11404285;61475143);国家高技术研究发展计划(批准号:2015AA020515)资助的课题~~

摘　　要：鉴于不同生理病理状态下组织复折射率实部的变化不大,传统光学相干层析(OCT)成像技术在分子特异性识别上存在先天不足.为此,本文提出了基于受激辐射信号的OCT成像方法,可在实现传统散射成像的同时,实现基于受激辐射信号的分子成像.在超高分辨率谱域OCT系统的基础上,通过增设光谱分光与调制抽运光支路,建立了基于单宽谱光源的抽运探测谱域OCT系统,详细推导了调制抽运下受激辐射信号的获取与成像公式.利用搭建的抽运探测谱域OCT系统,实现了瞬态受激辐射信号的相干探测.基于同时获取的受激辐射OCT信号和传统OCT信号,成功重构了氮化物粉末构建样品的基于受激辐射信号的分子对比OCT图像.Due to unnoticeable changes in complex refractive index of tissue under varied pathological and physiological states, the traditional optical coherence tomography（OCT） is deficient in molecular characterization. In this paper,the stimulated-emission based optical coherence tomography is proposed, which provides both molecular contrast and scattering contrast OCT imaging simultaneously. Based on the established ultra-high resolution spectral domain OCT system, a pump-probe spectral domain OCT system with a single wide-bandwidth light source is developed through an added modulated pump beam via spectrum splitting. In addition, the theory about the stimulated-emission signal and the image formulation under the modulated pump beam is presented. The coherent detection of the transient stimulated emission is realized by the developed pump-probe spectral domain OCT system. With the stimulated-emission OCT signal and the traditional OCT signal obtained at the same time, molecular contrast OCT images of the samples consisting of nitride powder are reconstructed successfully.

关 键 词：光学相干层析成像 受激辐射 抽运探测 分子特异性 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]