利用均匀设计法优化天麻萌发菌小菇属真菌(Mycena sp.)培养基的研究  

Optimization of Culture Media for the Growth of Seed-germinating Fungus Mycena sp.of Gastrodia elata Using the Uniform Design Method

作　　者：王玉川 秦丽媛 何海艳 陈林[2,3,4] 杨明挚 张汉波[2,4] WANG Yu-chuanl;QIN Li-yuan;HE Hai-yan;CHEN Lin;YANG Ming-zhi;ZHANG Han-bo(Gastrodia Tuber Research Institute of Zhaotong,Zhaotong 657000,China;School of Life Sciences,Yunnan University,Kunming 650091,China;School of Ecology and Environmental Science,Yunnan University,Kunming 650091,China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650091,China)

机构地区：[1]昭通市天麻研究院,云南昭通657000 [2]云南大学生命科学院,云南昆明650091 [3]云南大学生态与环境科学学院,云南昆明650091 [4]省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091

出　　处：《中国食用菌》2018年第5期23-28,31,共7页Edible Fungi of China

基　　金：国家自然科学基金(NO.31360153);2015年度昭通天麻共生优良"两菌"选育项目(NO.201501)

摘　　要：小菇属真菌(Mycena sp.)是天麻种植的重要萌发菌,系统研究小菇属真菌的营养生长因子需求,对天麻产业具有重要应用价值。本研究以小菇属真菌菌落直径和生物量同时增加作为优化目标,利用均匀设计法对影响真菌生长的14个营养因子进行配比优化,获得的最优培养基配方为:可溶性淀粉4 g·L^(-1)、蛋白胨4 g·L^(-1)、酵母膏4 g·L^(-1)、甘露醇8 mg·L^(-1)、烟酸4 mg·L^(-1)、 KH_2PO_41.6 g·L^(-1)、 MgSO_4·7H_2O 1.6 g·L^(-1)。在25℃, pH5.5培养21d,菌落直径达到69 cm,生物量为0.2685 g。可溶性淀粉和KH_2PO_4,蛋白胨和酵母膏间的交互作用对菌落直径有显著影响;蛋白胨和MgSO_4·7H_2O,甘露醇和MgSO_4·7H_2O间的交互作用对生物量大小有显著影响。研究结果表明影响小菇属真菌生长的营养因子较多,且其多被因子间的交互作用影响。Mycena species are important fungi for the germination of orchid Gastrodia data. Therefore it is valuable for G. elata production to understand the nutritional requirement of Mycena sp. growth. In this study, taking the colony diameter and biomass of Mycena sp. as the target, the ratio of 14 nutritional factors affecting the growth of fungi were optimized using the uniform design method, and the optimal formula is： soluble starch g g＂ L-l, peptone 4 g＂ L-1, yeast extract 4 g＂ L-1, mannitol 8 rag＂ L-l, nicotinic acid 4 mg.L-1, KH2P04 1.6 g＇L-1 and MgSO4＂7H20 1.6 g＇L-1. The interactions between KH2P04 and soluble starch, yeast extract and peptone had significant effect on the colony diameter of Mycena sp.. The interactions between peptone and MgSO4＂7H20, mannitol and MgSO4＂7H20 had significant effect on the biomass of Mycena sp.. The results showed that there were many nutrition factors affecting the growth of Mycena sp., and they were affected by the interaction between the factors.

关 键 词：均匀设计 营养因子 小菇属真菌 生物量 菌落直径 

分 类 号：S646.9[农业科学—蔬菜学]