翘芩清肺剂对哮喘大鼠气道平滑肌细胞外信号调节激酶通路的影响  

Study on Effects of Qiaoqin Qingfei Formula on ERK Pathway in Airway Smooth Muscle Cell of Asthmatic Rat

作　　者：张秋玲[1] 韩超[1] 徐俊[2] 杨柳[2] 梁慧玲[1] 杨洁颖[1] Zhang Qiuling;Han Chao;Xu Jun;Yang Liu;Liang Huilingl;Yang Jieying(Guangzhou Hospital of Chinese Medicine,Guangzhou 510130,China;School of Pharmacy,Jinan University,Guangzhou 510632,China)

机构地区：[1]广州市中医医院,广州510130 [2]暨南大学药学院,广州510632

出　　处：《世界中医药》2018年第7期1709-1713,共5页World Chinese Medicine

基　　金：广州市中医药;中西医结合项目(20152A011012);广州市中医药;中西医结合项目(20172A010009);广东省中医药局科技项目(20171224)

摘　　要：目的:探讨翘芩清肺剂在改善哮喘气道炎症及其可能的作用机制。方法:选取雌性SD大鼠50只,制备哮喘大鼠模型并随机分成5组,即正常组、模型组、地塞米松组和翘芩清肺剂低、高剂量组。分别采用酶联免疫吸附试验(ELISA)测定支气管肺泡灌洗液(BALF)中白细胞介素-1(IL-1)、IL-5及干扰素-γ(IFN-γ)的含量,并观察肺组织病理学改变;用免疫组织化学染色法观察细胞外信号调节蛋白激酶(ERK)在肺组织内的表达,转化生长因子(TGF)-β1在支气管组织内的表达;用实时定量-聚合酶链反应(Real-time PCR)测定气道平滑肌组织中ERK mRNA含量。结果:模型组中ERK蛋白含量及mRNA水平,TGF-β1蛋白表达较正常组有显著增加(P<0.05);与模型组比较,各干预组均能明显降低ERK mRNA及TGF-β1蛋白水平(P<0.05)。结论:翘芩清肺剂可明显改善哮喘大鼠的呼吸道症状,对气道具有保护作用,其作用机制可能与调节IL-4/IFN-γ平衡失调、抑制ERK通路的磷酸化,减轻气道炎症有关。Objective： To investigate the Qiaoqin Qingfei Formula in improving airway inflammation and its possible mechanism.Methods： Fifty female SD rats with asthma were randomly divided into 5 groups： normal group,model group,dexamethasone group and,low and high dose group of Qiaoqin Qingfei Formula.The contents of interleukin-1（ IL-1）,interleukin-5（ IL-5） and interferon gamma（ IFN-γ） in bronchoalveolar lavage fluid（ BALF） were detected with ELISA method after administration.The proteins expression of the Erk1/2 of the lung tissues and the TGF-β1 of the bronchial tissues were analyzed by Western blot method and the mRNA expression of ERK1/2 of the bronchial tissues were analyzed by Real-time PCR.Results： Compared with normal group,the mRNA expression of ERK and the protein expression of TGF-β1 were significantly increased（ P〈0.01）.Compared with the model group,all the treatment groups significantly decreased the expression of the ERK mRNA and the protein of TGF-β1（ P〈0.05-0.01）.Conclusion： Qiaoqin Qingfei Formula can significantly improve the symptoms of respiratory tract which has a protective effect on asthmatic rats.Its mechanism may be related to the inhibition of the phosphorylation of ERK signaling pathway,regulation of IL-4/IFN-γimbalance and reduction of inflammatory cell infiltration.

关 键 词：翘芩清肺剂 气道炎症 ERK信号通路 转化生长因子-Β1 

分 类 号：R289.5[医药卫生—方剂学]