红参提取物对D-半乳糖所致衰老小鼠的抗衰老作用及其机制研究  

Anti-Aging Effects and Mechanism of Red Ginseng Extract on D-Galactose Induced Aging Mice

作　　者：曾露露[1] 丁传波[1,2] 杨烁 佘欣鑫 刘兴龙 李自强[1] 吴钦超 刘文丛 郑毅男[1] ZENG Lu-lu;DING Chuan-bo;YANG Shuo;SHE Xin-xin;LIU Xing-long;LI Zi-qizng;WU Qin-ehao;LIU Wen-cong;ZHENG Yi-nan(College of Chinese Medicinal Materials,Jilin Agrieuhural University,Changehun 130118,China;Hunehun Huarui Ginseng Industry Bioengineering Co.,Ltd.,Hunehun 133300,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]珲春华瑞参业生物工程股份有限公司,吉林珲春133300

出　　处：《中国药学杂志》2018年第17期1470-1476,共7页Chinese Pharmaceutical Journal

基　　金：吉林省自然科学基金项目资助(20160101017JC);吉林省教育厅项目资助(吉教科合字[2016]第197号);吉林省科技发展计划重点科技攻关项目(20170204023NY);长春市科技创新(医药健康产业发展)重大专项资助(17YJ013)

摘　　要：目的探讨红参提取物(RG)对D-半乳糖(D-gal)所诱导的亚急性衰老小鼠模型的保护作用及与肿瘤坏死因子(TNF)-α、谷胱甘肽过氧化物酶(GPX) 1、GPX2、白细胞介素(IL)-1β、IL-6、IL-10的相关性。方法以D-半乳糖制备亚急性衰老小鼠模型,随机分为对照组、模型组、RG低、中、高剂量组(100,200,400 mg·kg-1)。造模同时给药42 d后,测定脾脏指数;比色法测定血清和脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)、血清中的乙酰胆碱酯酶(AChE)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)、诱导型一氧化氮合成酶(i-NOS); ELISA法测定脑组织中的环氧合酶-2 (COX-2)、TNF-α、IL-1β及IL-6的水平;实时荧光定量逆转录-聚合酶链反应(RT-PCR)检测脑组织中TNF-α、GPX1、GPX2、IL-1β、IL-6及IL-10 mRNA表达。结果 RG能增强衰老模型小鼠的脾脏指数,提高血清和脑组织中的SOD、血清中的AChE、CAT、GSH-PX活性,降低血清和脑组织中MDA,及血清中iNOS和脑组织中COX-2、TNF-α、IL-1β及IL-6的含量。同时明显抑制小鼠脑组织中TNF-α、IL-1β及IL-6的mRNA表达量,增加脑组织中GPX1、GPX2及IL-10的mRNA表达量,且具有统计学意义。结论 RG具有明显的抗衰老作用,其机制可能与TNF-α、GPX1、GPX2、IL-1β、IL-6及IL-10表达、增强机体免疫能力、清除自由基和炎症因子有关。OBJECTIVE To investigate the protective mechanism of red ginseng extract （RG） on aging mice induced by D- galactose and the correlation between tumor necrosis factor-α （TNF-α） , glutathione peroxidase 1 （ GPX1 ） , glutathione peroxi- dase2 （GPX2）, interleukin-1β （IL-1β）, interleukin-6 （IL-6） and interleukin-10 （IL-10）. METHODS Aging mice models were caused by D-galactose and randomly divided into control group, model group, RG low, medium and high dose （ 100, 200, 400 mg · kg- 1） groups. The preparation of model and drug delivery, after 42 d, the mice were killed and determination of spleen index; the activity of superoxide dismutase （SOD） and malondialdehyde （MDA） in serum and brain tissue were meas- ured. In serum, the levels of acetylcholinesterase （ACHE） , catalase （CAT） , glutathione peroxidase （GSH-PX） , inducible nitric oxide synthase （i-NOS） were measured. Enzyme-linked immunosorbent assay （ELISA） was used to test the levels of cyclooxygenase-2 （COX-2） , TNF-α, IL-1β and IL-6 in brain tissue. Real time quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to detect the correlation of TNF-α, GPX1, GPX2, IL-1 β, IL-6 and IL-10 gene mRNA expression in brain tissue. RESULTS The RG can enhance the spleen index, increase the activity of SOD in serum and brain tissue and ACHE, CAT and GSH-PX in serum, decrease the content of MDA in serum and brain tissue, and decrease the content of iNOS in serum and the content of COX-2, TNF-α, IL-1 β and IL-6 in brain tissue. At the same time, it significantly inhibited the mRNA expression of TNF-α, IL-1β and IL-6 in the brain tissue of mice, and increased the expression of GPX1, GPX2 and IL-110 mRNA in the brain tissue, and had statistical significance. CONCLUSION Concentrated solution of red ginseng extract could signifi-cantly exhibit anti-aging effects, its mechanism may be related to the TNF-α, GPX1, GPX2, IL-1 β, IL-6 and IL-10 expression in aging, enhancing immune

关 键 词：红参 抗衰老 肿瘤坏死因子-α 谷胱甘肽过氧化物酶1 谷胱甘肽过氧化物酶2 白细胞介素-1Α 白细胞介素-6 白细 胞介素-10 

分 类 号：R965[医药卫生—药理学]