白纹伊蚊雌蚊唾液腺匀浆血小板聚集抑制与抗凝血活性的探讨  

PRELIMINARY STUDIES ON PLATELET AGGREGATION INHIBITION AND ANTI-COAGULANT ACTIVITY IN SALIVARY GLANDS EXTRACT OF AEDES ALBOPICTUS

作　　者：李世琪 闫妍 翟慧 王政艳 程金芝 吴家红 LI Shi-Qi;YAN Yan;ZHAI Hui;WANG Zheng-Yan;CHENG Jin-Zhi;WU Jia-Hong(Department of Parasitology,Guiyang Medical University,Guiyang 550000,China;Department of Dermatology,General Hospital of Huaibei miner,Huaibei 235000)

机构地区：[1]贵州医科大学环境污染与疾病监控省部共建教育部重点实验室贵州医科大学现代病原生物学实验室贵州医科大学基础医学院,贵州贵阳550025 [2]淮北矿工总医院皮肤科,安徽淮北235000

出　　处：《寄生虫与医学昆虫学报》2018年第2期87-91,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目(No.81060138;81260260);贵州省科技基础平台建设项目(黔科合[2012(4006)]

摘　　要：本文旨在探讨白纹伊蚊Aedes albopictus唾液腺匀浆粗蛋白(salivary gland extract,SGE)血小板聚集抑制和抗凝血活性,为后续单个唾液蛋白在蚊虫吸血中的功能研究奠定基础。采用比浊法检测低、中、高浓度SGE在不同血小板聚集激活剂作用下对人血小板聚集活性的影响,手工法检测不同浓度SGE对人全血部分凝血活酶时间,凝血酶原时间,凝血酶时间的影响。结果显示,低浓度SGE对Ⅰ型胶原诱导的血小板聚集有显著抑制作用,随着浓度升高具有明显的量效关系,最高抑制率可达90. 36%;高浓度SGE对凝血酶诱导的血小板聚集具有显著抑制作用。SGE对ADP诱导的血小板聚集无明显抑制作用。SGE对人血浆APTT、PT、TT均有延迟效应,1. 25 ng/m L SGE即对TT有显著延迟效应。研究结果表明,白纹伊蚊SGE不仅对胶原诱导的血小板聚集有明显的抑制作用,而且可作用于血液级联凝集全过程。The platelet aggregation inhibition and anti-coagulant activity were detected to evaluate the functions of salivary gland extracts （SGE） of Ae. albopietus. The effect of platelet aggregation inhibition was monitored by turbidimetry with low, medium and high concentrations of SGE when induced by type I collagen, thrombin and ADP, respectively. The platelet aggregation induced by type I collagen was inhibited obviously by low concentrate SGE （ P 〈 0.05） with significant dose- effect relationship as the concentration increased, the highest inhibition rate was 90% ; the platelet aggregation induced by thrombin was inhibited significantly by high concentrate SGE （P 〈 0. 05 ） , SGE showed no obvious inhibition effect on platelet aggregation induced by ADP. Under certain concentration of SGE, APTT, PT and TT extended significantly （P 〈 0.01 ） , and TT was extended at 1.25 ng/mL SGE （ P 〈 0.05 ）. The results showed that SGE has significant inhibition activity on platelet aggregation induced by collagen and can interact at all levels of coagulation （the intrinsic, extrinsic and common pathways）.

关 键 词：白纹伊蚊 唾液腺匀浆 血小板聚集 抗凝血 

分 类 号：R384.1[医药卫生—医学寄生虫学]