rDNA-ITS序列在吸血蠓分子鉴定中的应用研究  

APPLICATION OF rDNA-ITS GENE SEQUENCE IN MOLECULAR IDENTIFICATION OF BITING MIDGES

作　　者：王崇财 陈敏[2] 杨美琼[2] 潘林奇 云小云 黄恩炯[2] WANG Chong-Cai;CHEN Min;YANG Mei-Qiong;PAN Lin-Qi;YUN Xiao-Yun;HUANG En-Jiong(Hainan International Travel Healthcare Center,Haikou,Hainan 570100,China;Fujian International Travel Healthcare Center,Fuzhou,Fujian 350001,China)

机构地区：[1]海南国际旅行卫生保健中心,海南海口570100 [2]福建国际旅行卫生保健中心,福建福州350001

出　　处：《寄生虫与医学昆虫学报》2018年第2期92-100,共9页Acta Parasitologica et Medica Entomologica Sinica

基　　金：原国家质检总局科技计划项目(2016IK098;2016IK034;2015IK036);福建省重点科技项目(2014I0001)

摘　　要：以海南、福建等省常见库蠓为研究对象,构建基于ITS的吸血库蠓成虫分子鉴定体系。共24种库蠓的72条序列用于比较分析、遗传距离和系统发育分析显示:24种库蠓的种间遗传距离为0. 05～0. 39,平均遗传距离0. 25;种内遗传距离为0～0. 13,平均遗传距离0. 07。种间遗传距离为种内遗传距离的3. 57倍,表明ITS基因具有较大的种间甚至种内变异性,是理想的靶标基因,可作为库蠓种类鉴定的分子标记。Specimens of biting midges collected from Hainan, Fujian, Guangxi, Heilongjiang and Xinjiang province and autonomous region were studied to construct the molecular identification system of adult Culicoides. Twenty-four species of Culicoides adults and 72 ITS gene sequences were analyzed. It was showed from genetic distance and by phylogenetic analysis that the interspecifie genetic distance was 0.05 - 0. 39 with average distance of 0.25, and the intraspecifie genetic distance was 0 - 0. 13 with average distance of 0.07. The interspecific genetic distance was 3.57 times of intraspecific genetic distance, indicating that ITS genes might be employed as one of suitable molecular markers for identifying the bloodsucking midges.

关 键 词：分子鉴定 ITS1 ITS2 库蠓 

分 类 号：R384.5[医药卫生—医学寄生虫学]