和中健脾方对混合反流性食管炎模型大鼠食管黏膜的保护作用及对核转录因子-кB信号通路的影响  

Protective effects of Hezhong Jianpi Decoction on esophageal mucosa of rats with mixed reflux esophagitis and its effect on nuclear transcription factor-κB signaling pathway

作　　者：肖姣 李亦凡 罗斌[2] 李莹杰[2] 吴萍[2] 朱凌云[1] XIAO Jiao;LI Yifan;LUO Bin;LI Yingjie;WU Ping;ZHU Lingyun(Department of Gastroenterology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;Department of Clinical Nutrition,Tongfi Hospital Affiliated to Tongfi University,Shanghai 200065,China)

机构地区：[1]上海中医药大学附属市中医医院消化内科,上海200071 [2]同济大学附属同济医院临床营养科,上海200065

出　　处：《上海中医药杂志》2018年第8期73-77,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市卫计委资助项目(2014LP078A);上海市进一步加快中医药事业发展三年行动计划(2014-2016年)建设项目(ZY3-CCCX-1-1009);市级医院新兴前沿技术联合攻关项目(SHDC12016212)

摘　　要：目的观察和中健脾方对混合反流性食管炎模型大鼠食管黏膜损伤的保护作用及对介导炎性反应的核转录因子-кB(NF-кB)信号通路的影响。方法 60只健康SPF级SD雄性大鼠采用随机数字表法分为正常组、假手术组、模型组、和中健脾方组及西药奥美拉唑组,每组12只。建立混合反流性食管炎大鼠模型,造模3天后给予药物灌胃14天。观察食管组织形态学病理改变并评分,同时检测比较大鼠血清白介素-6(IL-6)、白介素-8(IL-8)、NF-кB p65蛋白的表达。结果模型组大鼠食管黏膜肉眼积分、病理积分、血清NF-кB p65、IL-6、IL-8表达较正常组、假手术组、和中健脾方组及奥美拉唑组均明显升高(P<0.05);和中健脾方组大鼠食管黏膜肉眼、病理积分与奥美拉唑组比较差异无统计学意义(P>0.05);和中健脾方组血清NF-кB p65、IL-6、IL-8表达高于奥美拉唑组(P<0.05)。结论和中健脾方能降低反流性食管炎大鼠食管黏膜的病理损伤程度,其作用机制可能是通过下调模型大鼠血清中促炎蛋白IL-6、IL-8、NF-кB p65的表达水平,从而减轻食管炎症反应达到抗炎作用。Objective To observe the protective effects of Hezhong Jianpi Decoction on the esophageal mucosal injury in rats with mixed reflux esophagitis and its effects on nuclear transcription factor-кB（NF-кB） signaling pathway,which mediated the inflammatory response. Methods Sixty healthy male SD rats of SPF grade were randomly divided into the normal group,sham operation group,model group,Hezhong Jianpi Decoction group and omeprazole group according to the random number table,12 rats in each group. The rat model of mixed reflux esophagitis was established. 3 days after modeling,the rats were treated the corresponding drug by intragastric administration for 14 days. The histopathological changes of esophageal tissues were observed and scored. Meanwhile,the serum levels of interleukin-6（IL-6）,IL-8 and NF-кB p65 were detected. Results The naked eye and pathological scores of esophageal mucosal and the serum expressions of NF-кB p65,IL-6,and IL-8 in the model group were significantly higher than those in the normal group,sham operation group,Hezhong Jianpi Decoction group and omeprazole group（P〈0.05）. There were no statistically significant differences on the naked eye and pathological scores of esophageal mucosal between the Hezhong Jianpi Decoction group and the omeprazole group（P〈0.05）. The serum expressions of NF-кB p65,IL-6,and IL-8 in the Hezhong Jianpi Decoction group were higher than those in the omeprazole group（P〈0.05）. Conclusion Hezhong Jianpi Decoction can reduce the pathological damage degree of esophageal mucosa in rats with reflux esophagitis. Its mechanism may be related to down-regulating the serum expression levels of proinflammatory cytokines IL-6,IL-8 and NF-кB p65 in model rats,and thus relieving the esophageal inflammatory response to exert anti-inflammatory effects.

关 键 词：和中健脾方 反流性食管炎 核转录因子-КB 白介素-6 白介素-8 大鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]