水中粪大肠菌群检测方法的对比  

Comparison of Detection Methods of Fecal Coliforms in Water

作　　者：何敏[1] 张亚娟 周艳红 余沛芝 周婷 董晓晨[1] 赵赛君[1] 邹真妮 Yun Yunbo 刘洪波[2] He Min;Zhang Yajuan;Zhou Yanhong;Yu Peizhi;Zhou Ting;Dong Xiaochen;Zhao Saijun;Zou Zhenni;Yun Yunbo;Liu Hongbo(Water Quality Detection Center of Suzhou Industrial Park Qingyuan ＆ Hongkong China Water Co.,Ltd.,Suzhou 215021,China;School of Environment and Architecture,University of Shanghai for Science and Technology,Shanghai 200093,China;The Research Institute for Water and Waste Management,RWTH Aachen University,Aachen 52056,Germany)

机构地区：[1]苏州工业园区清源华衍水务有限公司水质检测中心,江苏苏州215021 [2]上海理工大学环境与建筑学院,上海200093 [3]亚琛工业大学水和固体废物管理研究所,德国亚琛52056

出　　处：《净水技术》2018年第9期19-22,共4页Water Purification Technology

基　　金：比尔及梅琳达盖茨基金会资助项目(RTTC-2014);水处理技术研究以及相关领域虚拟合作平台创建协议资助项目(201608);中德深度去除污水养分技术合作共同协议资助项目(201709);中德深度去除污水养分技术研究(201608-2)

摘　　要：文中进行了多管发酵法和酶底物法检测水中(耐热)粪大肠菌群结果的对比。首先,试验通过对认证的商售IDEXXQC大肠埃希氏菌和绿脓假单胞菌分别作为阳性和阴性控制菌株进行检测,其结果显示均在置信范围。其次,用较清洁的水样和污水进水水样分别进行多管发酵法和酶底物法平行检测,并进行配对t检验。较清洁的水样结果显示P=0.818(>0.05)、相关系数r=0.947;污水进水水样结果显示P=0.712(>0.05)、相关系数r=0.602,差异无统计学意义。相关系数均大于0.6,两种检测方法相关性均较强,说明多管发酵法和酶底物法检测水中(耐热)粪大肠菌群具有一致性。This paper focused on the comparison of detection results of （heat-resistant） fecal coliforms in water with multi-tube fermentation and enzyme substrate. Firstly, the test was carried out by certified commercial IDEXX-QC Escherichia coli and Pseudomonas aeruginosa using as positive and negative control strains respectively, whose results were in the confidenee range. Secondly, the cleaner water samples and wastewater influent water samples were tested by multi-tube fermentation method and enzyme substrate method respectively, and paired t test was carried out. Results of the cleaner water samples showed that the correlation coefficient （ r = 0. 947） was 0. 818 （ 〉 0. 05）, while wastewater sample was 0. 712（ 〉0. 05）, r=0. 602. There was no statistical significance in explaining the difference. Correlation coefficients were all greater than 0. 6, which indicated that multi-tube fermentation method and enzyme substrate method were consistent in the detection of fecal coliform bacteria in water.

关 键 词：多管发酵法 酶底物法(耐热) 粪大肠菌群 

分 类 号：X832[环境科学与工程—环境工程]