猪伪狂犬病毒gE基因缺失毒株的构建及其免疫原性的研究  

Construction and immunogenicity of gE gene deletion mutant of Pseudorabies virus

作　　者：林鸷[1] 何锡忠[1] 李本强[1] 潘洁[1] 朱永军[1] 赵本进[1] 彭丽英[1] LIN Zhi;HE Xi-zhong;LI Ben-qiang;PAN Jie;ZHU Yong-jun;ZHAO Ben-jin;PENG Li-ying(Institute of Animal Husbandry ＆ Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 21106,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《上海农业学报》2018年第4期100-103,共4页Acta Agriculturae Shanghai

基　　金：上海市农业委员会重点项目[沪农科攻字(2015)第1-7号];上海市农业科学院助推项目(ZT2018009);上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-20号]

摘　　要：通过CRISPER/Cas9编辑技术编辑实验室分离得到的PRV-SX株的gE基因,并利用蚀斑纯化等方法得到gE基因缺失的突变株。通过T7E1核酸内切酶及测序来鉴定gE基因的缺失,并对gE基因缺失毒株的免疫效果进行了研究。结果表明:应用CRISPER/Cas9编辑技术成功得到了gE基因缺失毒株,用这一毒株制备疫苗免疫仔猪,免疫后0 d、14 d、28 d、42 d、56 d PRV gE抗体均为阴性;PRV gB抗体呈阳性,在42 d时达高峰。In this study,the CRISPER/Cas9 editing technique was used to target the gE gene of the epidemic strain PRV-SX. The gE gene deletion mutant was obtained by means of plaque purification. The deletion of gE gene was identified by T7 E1 endonuclease and sequencing. Subsequently, the immune effect of gE gene deletion strain was also studied. The results showed that a gE gene-deficient strain was successfully obtained by using the CRISPER/Cas9 editing technology. After piglets being inoculated with the vaccine,the PRV gE antibody in them was negative at 0 d,14 d,28 d,42 d and 56 d after vaccination. Meanwhile,the PRV gB antibody was positive and reached the peak at 42 d.

关 键 词：伪狂犬病毒 gE基因缺失 免疫原性 

分 类 号：S858.28[农业科学—临床兽医学]