大鼠失眠模型造模中对氯苯丙氨酸混悬液改良法的探讨  

An Improved Method for the Preparation of Para-chlorobenzalanine Suspension in a Rat Model of Insomnia

作　　者：魏歆然 郑雪娜[2] 裴芸[2] 吴雪芬[2] 刘丽[2] 陈小丽[2] 文琪琦[2] 岳增辉[2] 魏高文[2] WEI Xinrarcn;ZHENG Xuena;PEI Yun;WU Xuefen;LIU Li;CHEN Xiaoli;WEN-Qiqi;YUE Zenghui;WEI Gaowen(The First Affiliated Hospital of Hunan Universty of Ch#wse Medicine,Changsha,Hunarz 41000Z China;Hunan College of Chinese Medicine,Changsha,Hunarl 41020＆ China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2018年第9期986-989,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2014CB543102)

摘　　要：目的探讨大鼠失眠模型造模中对氯苯丙氨酸(para-chlorophenylalanine,PCPA)混悬液配制的改良方法,并对造模效果进行评价。方法 98只SD大鼠中50只用NaOH溶液配制法、24只用NaHCO_3溶液配制法和24只用改良法进行失眠大鼠造模,探讨制作PCPA致失眠模型的最佳方法。结果 NaOH溶液配制法50只大鼠造模失败,NaHCO_3溶液配制法24只大鼠造模失败,改良法24只大鼠造模成功,且PCPA剂量以0.5 g/kg造模效果最好(P<0.05)。结论改良的PCPA混悬液的配制法可以成功制作大鼠失眠模型。Objective To explore the improved method of preparing para-chlorophenylalanine （PCPA） suspension in a rat model of insonmia, and evaluate the eftect of modeling. Methods In the 98 SD rats, 50 rats were treated with NaOH solution preparing method, 24 SD rats were modeled with NaHC03 solution preparing method, and 24 SD rats were treated with the improved method to establish the model of insonmia rats. Results There were 50 tailed model rats with NaOH solution preparing method, 24 tailed model rats with NaHC03 solution preparing method. The 24 model rats succeed by the improved method, and the 0.5 g/kg of PCPA was the best dose to establish the model （P〈0.05）. Conclusion The improved preparing method of PCPA suspension can successfully make the rat model of insomnia.

关 键 词：对氯苯丙氧酸 失眠 造模 

分 类 号：R332[医药卫生—人体生理学]