拟南芥图位克隆快速初定位系统的建立  

Establishment of Rapid Initial Localization System of Arabidopsis Based on Map-based Cloning

作　　者：阚东阳 柯学[2] Walid Ghidan 肖素勤[2] 肖卿 胡向阳[4] 孔祥祥 黄兴奇[2] 程在全 KAN Dong-yang;KE Xue;Walid Ghidan;XIAO Su-qin;XIAO Qin;HU Xiang-yang;KONG Xiang-xiang;HUANG Xing-qi;CHENG Zai-quan(College of Agronomy and Biotechnology,Yunnan Agricultural University,Yunnan Kumning 650201,China;Biotechnology and Genet-ic Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Key Lab of Yunnan Provincial Agricultural Biotechnology Key Lab of Southwestern Crop Gene Resources and Germplasm Innovalion,Ministry of Agriculture,Yunnan Kunming 650201,China;Yunnan Blue biao Commeilcal and Trading Co.,Yunnan Huize 650420,China;Institute of Tibetan Plateau at Kunming,Chinese Acade my of Sciences,Yunnan Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南作物基因资源与种质创制重点实验室,云南昆明650223 [3]云南蓝虑商贸有限公司,云南会泽650420 [4]中国科学院昆明植物研究所,云南昆明650201

出　　处：《西南农业学报》2018年第9期1765-1771,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31460054;31401707);NSFC-云南联合基金(U1302265);云南省基金面上项目(2018FB035)

摘　　要：【目的】拟南芥是研究植物基因功能的工具材料,在图位克隆进行基因定位时面对数千个分子标记常常让新的研究者无从下手。【方法】本文通过从先前筛选的44个分子标记,进一步筛选出了24个平均分布于拟南芥五条染色体上的遗传标记。【结果】这24个标记在PCR检测中易于扩增、实验条件相差小、电泳条带区分度好,是进行图位克隆的理想分子标记。【结论】利用这些标记,建立了拟南芥初定位快速图位克隆系统,大大提高了初定位的效率,并实现拟南芥盐敏感突变体S28的快速染色体初定位。[ Ohjective]Arabidopsis thaliana was a tool for studying the function of plant genes. It was often difficult for new researchers to figure out thousands of molecular markers when mapping genes for positional cloning. [ Method] In this study, 24 genetic markers distributed on five chromosomes of Arabidopsis thaliana were screened out by using 44 molecular markers screened previously. [ Result] These 24 markers were easy to amplify in PCR detection, the experimental condition was relatively consistent, and the electrophoresis bands divisions were good, and it was an ideal molecular marker for map cloning. [ Conclusion] Using these markers, fast cloning system of the Arabidopsis was established, which greatly improved the efficiency of initial positioning, and achieved rapid initial localization of chromosome of Arabidopsis salt sensitive mutants S28, had proved that the method was efficient and practical.

关 键 词：拟南芥 图位克隆 突变体 初定位 遗传标记 

分 类 号：Q943[生物学—植物学]