口服湿热痹胶囊大鼠体内代谢产物分析  

Identification of the metabolites in rats after oral administration of Shirebi capsule

作　　者：李鑫 杨钻 潘韬文[2] 王嘉一 丁雪 李坤[1] LI Xin;YANG Zhuan;PAN Taowen;WANG Jiayi;DING Xue;LI Kun(College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian,Liaoning Province,116029;Institute of Integrative Medicine,Dalian Medical University,Dalian,Liaoning Province,116044,China)

机构地区：[1]辽宁师范大学化学化工学院,辽宁大连116029 [2]大连医科大学中西医结合学院,辽宁大连116044

出　　处：《吉林医药学院学报》2018年第5期330-333,共4页Journal of Jilin Medical University

摘　　要：目的鉴定湿热痹胶囊给予大鼠后血浆、尿液和粪便中的代谢产物。方法色谱柱为Waters C18(2.1 mm×50 mm,1.7μm),柱温40℃。流动相:0.1%甲酸水溶液-0.1%甲酸乙腈溶液,梯度洗脱。超高压液相色谱-飞行时间质谱仪采用电喷雾电离离子源,正、负离子模式检测。结果超高效液相色谱-质谱联用法分析出湿热痹胶囊中7种入血的原型成分,在尿液中分析出湿热痹胶囊原型成分6种,粪便中分析出湿热痹胶囊原型成分11种。结论体内测得成分可能为湿热痹胶囊发挥药效的物质基础。Objective To identify the metabolites in rat plasma,urine and faces after oral administration of SHIREBI capsule. Methods Column of Waters C 18 （2.1 mm × 50 mm,1.7 μm） was used at 40 ℃,folic acid contained water and acetonitrile was used as mobile phase in a gradient elution.Analysis of HPLC-QTOF MS with electrospray ion source was conducted in both positive and negative mode. Results Seven,six and eleven kinds of metabolites were identified by the proposed method. Conclusion The prototype metabolites found in plasma maybe the effective compounds in Shirebi capsule.

关 键 词：湿热痹胶囊 超高压液相色谱-飞行时间质谱仪 代谢产物 

分 类 号：R286[医药卫生—中药学]