感染黄龙病菌亚洲柑橘木虱基因表达的转录组学分析  

Transcriptomic analyses of Diaphorina citri Kuwayama in response to the infection by Candidatus Liberibacter asiaticus

作　　者：曾丽霞 田明义[1] 吴丰年[1] 王妍晶[1] 邓晓玲[1] 岑伊静[1] ZENG Li-Xia;TIAN Ming-Yi;WU Feng-Nian;WANG Yan-Jing;DENG Xiao-Ling;CEN Yi-Jing(College of Agriculture,South China Agricultural University,Guangzhou 510642,China;Guangdong Lingnan Vocational and Technical College,Guangzhou 510663,China)

机构地区：[1]华南农业大学农学院,广州510642 [2]广东岭南职业技术学院,广州510663

出　　处：《应用昆虫学报》2018年第4期574-585,共12页Chinese Journal of Applied Entomology

基　　金：现代农业(柑桔)产业技术体系项目(CARS-27);公益性行业(农业)科研专项(201003067-01);国家重点研发计划项目(SQ2018YFD020103)

摘　　要：【目的】了解亚洲柑橘木虱Diaphorina citri Kuwayama与黄龙病菌Canditatus Liberibacter asiaticus的分子互作机制。【方法】利用转录组(RNA-Seq)方法分别对携带和未携带黄龙病菌的柑橘木虱成虫进行转录组测序,根据获得的转录信息分析木虱携带黄龙病菌后基因表达的差异。【结果】获得了1 502个差异表达的unigenes,在带菌木虱中上调和下调的基因分别有746和756个。共有1 099个unigenes比对上NCBI蛋白数据库并获得功能注释;852个被聚类到GO的三大功能中;931个被注释到KEGG的239个代谢通路中。根据差异表达基因的GO分析,木虱代谢过程和催化活性的相关基因明显上调。此外,筛选出53个免疫相关的unigenes中,28个与细胞免疫、25个与免疫信号路径相关,分别有64%和32%表达上调。【结论】黄龙病菌和媒介昆虫柑橘木虱存在互作关系,病菌入侵后可能通过代谢活动相关基因的上调和免疫相关基因的差异表达而影响木虱的代谢活动和免疫反应过程。[Objectives] Understanding of the molecular mechanism of interaction between Asian citrus psyllid Diaphorina citri Kuwayama and huanglongbing which is associated with Canditatus Liberibacter asiaticus（CLas）. [Methods] RNA-Seq technology was used to analyze the transcriptome of CLas infected and uninfected D. citri adults. The differentially expression genes after CLas-infection were obtained, and their functions were analyzed. [Results] There were 1 502 unigenes that were differentially expressed between the infected and uninfected D. citri, with 764 up-regulated and 752 down-regulated in the infected psyllids. These unigenes were assigned to NCBI NR, KEGG and GO database, 1 099 unigenes had significant Blast X hit with NCBI protein database and their annotations of predicted protien were obtained. Among them, 852 unigenes were categorized into GO function categories; 931 unigenes were categorized into 239 KEGG pathways. According to the GO analysis, the unigenes which involved in metabolic process and catalytic activity function were significantly up-regulated. Among the 53 immune-related genes which showed different express, 28 were related to the cellular immunity, 25 to the immune signaling pathway, and the up-regulated genes of these 2 groups was 64% and 32% respectively. [Conclusion] Interaction is present between CLas and it＇s vector D. citri. The metabolic activity and the immunoreaction might be affected as the related genes of these activity differently express in the CLas-infected psyllid.

关 键 词：亚洲柑橘木虱 黄龙病 转录组 差异表达 互作 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]