家蚕幼虫咽侧体体积变化分析  

Change in the corpus allatum of silkworm, Bombyx mori, larvae

作　　者：李亚腾 张亮[1] 李田[1] 王小康 赵威[1] 陈萍[1,2] LI Ya-Teng;ZHANG Liang;LI Tian;WANG Xiao-Kang;ZHAO Wei;CHEN Ping(College of Biotechnology,Southwest University,Chongqing 400715,China;Agriculture Ministry Key Laboratory of Sericulture Biology ＆ Genetic Breeding,Chongqing 400715,China)

机构地区：[1]西南大学生物技术学院,重庆400715 [2]农业部蚕桑生物学与遗传育种重点实验室,重庆400715

出　　处：《应用昆虫学报》2018年第4期698-704,共7页Chinese Journal of Applied Entomology

基　　金：重庆市基础研究与前沿探索(cstc2018jcyj AX0075);重庆市2018年度茧丝绸发展补助资金项目

摘　　要：【目的】本试验旨在研究不同生长发育时期家蚕幼虫咽侧体及其腺细胞的变化。【方法】选取家蚕4眠蚕中限品种作为实验材料,对其4龄、5龄等时期进行解剖处理,得到咽侧体后进行透析处理与DAPI染色,在荧光显微镜下成荧光像图,结合软件分析工具测量咽侧体横截面积直径与细胞数量来确定家蚕咽侧体及其腺细胞在不同生长时期的变化。【结果】咽侧体体积随幼虫生长发生了明显增长;咽侧体细胞数目随幼虫生长会增加,但比较缓慢。随着幼虫的生长,腺细胞体积会增大,同时咽侧体体积增长与腺细胞核体积增长有极显著相关性。【结论】家蚕咽侧体体积增长主要源于腺细胞体积的增大而稍带腺细胞数目的增加,家蚕幼虫咽侧体体积与生长发育的时间经过密切相关。[Objectives] To investigate changes in the corpus allatum and glandular cells of silkworm larvae at different stages of development. [Methods] The corpus allatum was dissected from fourth and and fifth instar larvae of the tetramolter Zhongxian variety of silkworm. Fluorescent images of the corpus allatum and glandular cells were obtained using a fluorescent microscope after dialysis treatment and DAPI staining and combined with an analysis tool to measure the cross sectional area of the corpus allatum and the number of glandular cells. [Results] The volume of the corpus allatum increased significantly as larvae developed. The number of glandular cells also increased with the development but this was relatively slow. As larvae developed, the volume of glandular cells increased, and the volume of the corpus allatum was significantly correlated with glandular cell size. [Conclusion] The increase in the volume of the corpus allatum in silkworm larvae is mainly due to the increased volume of glandular cells. The number of glandular cells and the volume of the corpus allatum is closely related to the developmental stage of larvae.

关 键 词：家蚕 咽侧体 腺细胞 体积 

分 类 号：S881.2[农业科学—特种经济动物饲养]