Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose  

作　　者：Kit Wayne Chew Pau Loke Show Yee Jiun Yap Joon Ching Juan Siew Moi Phang Tau Chuan Ling Jo-Shu Chang 

机构地区：[1]Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia [2]Bioseparation Research Group, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia [3]Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia [4]Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia [5]Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia [6]Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia [7]Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan, China

出　　处：《Frontiers of Environmental Science & Engineering》2018年第4期17-23,共7页环境科学与工程前沿（英文）

摘　　要：Various prctreatments methods including sonication and grinding were perfonned on red seaweed Gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEG） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.Various prctreatments methods including sonication and grinding were perfonned on red seaweed Gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEG） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.

关 键 词：AgaroseGe lidium PRETREATMENT SEAWEED Sonication 

分 类 号：TS727[轻工技术与工程—制浆造纸工程] Q948.885.3[生物学—植物学]