Mixed culture of Chlorella sp. and wastewater wild algae for enhanced biomass and lipid accumulation in artificial wastewater medium  

作　　者：Kishore Gopalakrishnan Javad Roostaei Yongli Zhang 

机构地区：[1]Department of Civil and Environmental Engineering, Wayne State University, Detroit, MI 48201, USA＊

出　　处：《Frontiers of Environmental Science & Engineering》2018年第4期141-156,共16页环境科学与工程前沿（英文）

摘　　要：The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions （i.e. CO2, light intensity, cultivation time, and inoculation ratio） for enhanced algal biomass and lipid productivity in wastewater medium using Response SuHhce Methodology （RSM）. The results show that mixed cultures ofd7llorella sp. and wastewater wild algae increase biomass and lipid yield. Additionally, findings indicate that CO2, light intensity and cultivation time significantly affect algal productivity. Furthcnnore, CO2 concentration and light intensity, and CO2 concentration and algal composition, have an interactive effect on biomass productivity. Under dii＂ferent cultivation conditions, the response of algal biomass, cell count, and lipid productlvlty ranges from2,5 to 10.2 mg/mL 1.1 × 10 to 8.2 × 10 cells/mL and 1.1 × 10^6 to 6.8 × 10^12 total fluorescent units/mL, respectively× The optimum conditions tbr simt, ltaneot, s biomass and lipid accumulation are 3.6% of CO2 （v/v）, 160 μmol/m^2/s of light intensity, 1×6/2.4 of inoculation ratio （wastewater-algae/Chlorella）, and 8.3 days of cultivation time. The optimal productivity is 9,8 （g/L） for dry biomass, 8.6 E ＋ 08 （cells/mL） for cell count, and 6.8 E ＋ 12 （Total FL units per mL） fbr lipid yield, achieving up to four times, eight times, and seven times higher productivity compared to non- optimized conditions. Provided is a supportive methodology to improve mixed algal culture for hioenergy feedstock generation and to optimize cultivation conditions in complex wastewater environments. This work is an important step tbrward in the development of sustainable large-scale algae cultivation for cost-efficient generation of biofuel.The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions （i.e. CO2, light intensity, cultivation time, and inoculation ratio） for enhanced algal biomass and lipid productivity in wastewater medium using Response SuHhce Methodology （RSM）. The results show that mixed cultures ofd7llorella sp. and wastewater wild algae increase biomass and lipid yield. Additionally, findings indicate that CO2, light intensity and cultivation time significantly affect algal productivity. Furthcnnore, CO2 concentration and light intensity, and CO2 concentration and algal composition, have an interactive effect on biomass productivity. Under dii＂ferent cultivation conditions, the response of algal biomass, cell count, and lipid productlvlty ranges from2,5 to 10.2 mg/mL 1.1 × 10 to 8.2 × 10 cells/mL and 1.1 × 10^6 to 6.8 × 10^12 total fluorescent units/mL, respectively× The optimum conditions tbr simt, ltaneot, s biomass and lipid accumulation are 3.6% of CO2 （v/v）, 160 μmol/m^2/s of light intensity, 1×6/2.4 of inoculation ratio （wastewater-algae/Chlorella）, and 8.3 days of cultivation time. The optimal productivity is 9,8 （g/L） for dry biomass, 8.6 E ＋ 08 （cells/mL） for cell count, and 6.8 E ＋ 12 （Total FL units per mL） fbr lipid yield, achieving up to four times, eight times, and seven times higher productivity compared to non- optimized conditions. Provided is a supportive methodology to improve mixed algal culture for hioenergy feedstock generation and to optimize cultivation conditions in complex wastewater environments. This work is an important step tbrward in the development of sustainable large-scale algae cultivation for cost-efficient generation of biofuel.

关 键 词：Algal biofuels Algal mixed cultures Algal biomass Algal lipid WASTEWATER Response surface methodology 

分 类 号：TQ658[化学工程—精细化工] Q-9[生物学]