玉米卷叶突变体rol1的遗传分析与基因定位  

Genetic Analysis and Low-resolution Mapping of Maize Mutant Rolled Leaf rol1

作　　者：刘慧 牟颖熙 王国英[1] 郑军[1] LIU Hui;MOU Ying-xi;WANG Guo-ying;ZHENG Jun(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《植物遗传资源学报》2018年第5期937-941,共5页Journal of Plant Genetic Resources

基　　金：中国农业科学院创新工程专项经费资助

摘　　要：叶型是作物理想株型育种的关键因素之一,发掘叶片发育相关基因对作物理想株型育种以及叶片发育的分子遗传机理研究具有重要意义。在玉米转座子Mutator活性系的杂交后代中,鉴定到一个玉米叶片卷曲的突变体,命名为rol1。突变体rol1在拔节期可明显观察到叶片内卷的表型,遗传分析表明,该卷叶表型受单隐性基因控制。将玉米骨干自交系B73和rol1杂交,构建遗传定位群体,利用BSR-Seq技术,对F2分离群体中的卷叶和展叶表型单株分别取样混池,进行转录组测序,将Rol1基因粗略地定位在玉米第5号染色体165~185 Mb区间内。进一步利用遗传分离群体中的卷叶表型单株缩小定位区间,将Rol1基因定位在SSR标记umc1822和umc1155之间,物理距离为5 Mb。本研究为Rol1基因的克隆和功能研究奠定了基础。Leaf shape is one of the factors important for crop architecture,and uncovering the genes that control the leaf shape development is of interest in breeding for ideal plant architecture and also in basic theory research. By screening the maize mutant library generated from Mutator active hybird lines,we identified a rolling leaf mutant rol1,which showed dramatic rolling phenotype on its leaves toward the adaxial side at the elongation stage. Genetic analysis indicated that the rolling leaf phenotype was controlled by a single recessive gene. F2 segregation population was constructed derived from a cross of inbred line B73 and rol1. The Rol1 gene was roughly mapped to the physical interval of 165-185 Mb on the chromosome 5 by BSR-Seq strategy,by sequencing the mutant and wild type pool derived from F2 population. Furthermore,we narrowed down the candidate region to a 5 Mb interval between SSR markers umc1822 and umc1155. Thus,these results provided useful and confident information for cloning and functional characterization of Rol1 gene in the future.

关 键 词：玉米 卷叶 突变体 基因定位 

分 类 号：S513[农业科学—作物学]