表没食子儿茶素没食子酸酯(EGCG)对肝硬化大鼠端粒酶逆转录酶表达的影响  

EFFECTS OF EGCG ON THE EXPRESSION OF TELOMERASE REVERSE TRANSCRIPTASE IN LIVER OF RATS WITH HEPATIC CIRRHOSIS

作　　者：宾文婷[1] 常加松[1] 史小林[2] BIN Wen-ting;CHANG Jia-song;SHI Xiao-lin(Department of Human Anatomy and Histoembryology,Nanjing University of Chinese Medicine,Nanjing 210023;1 Department of Histology and Embryology,Capital Medical University,Beijing 100069,China)

机构地区：[1]南京中医药大学人体解剖与组织胚胎学系,南京210023 [2]首都医科大学组织学与胚胎学教研室,北京100069

出　　处：《营养学报》2018年第4期360-365,370,共7页Acta Nutrimenta Sinica

基　　金：江苏省高校自然科学研究计划资助项目(No.13KJD310001)

摘　　要：目的探讨表没食子儿茶素没食子酸酯(Epigallocatechin gallate, EGCG)对肝硬化的治疗作用以及对其端粒酶活性的影响。方法将Wistar大鼠随机分为正常对照组跟造模实验组,造模实验组用改良的四氯化碳(carbon tetrachloride,CCl_4)法建立Wistar大鼠肝硬化模型;将实验组模型大鼠再随机分为模型组及EGCG组:模型组继续给予CCl_4,EGCG组继续给予CCl_4的同时,每日EGCG灌胃给药,各组均于8w后处死。测定各组肝功能,用苏木素和伊红(hematoxylin and eosin, HE)染色观察各组肝组织病理学改变,应用免疫组织化学方法测定各组肝组织的端粒酶逆转录酶(telomerase reverse transcriptase, TERT)的表达,用Real-time Polymerase Chain Reaction(Real-time PCR)测定各组肝组织TERT的mRNA的表达。结果模型组肝组织呈现典型肝硬化病理学改变,EGCG组肝纤维化程度较模型组明显减轻;血清生化指标丙氨酸氨基转移酶(alanineaminotransferase,ALT)跟天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)也较模型组明显降低;免疫组织化学结果显示TERT在模型组的表达高于正常对照组(P<0.01),TERT在EGCG组的表达低于模型组(P<0.05);EGCG组TERT的mRNA表达亦低于模型组(P<0.05)。结论 EGCG可减轻大鼠肝纤维化程度,可有效降低肝硬化大鼠肝组织TERT的表达水平。Objective To study the efficacy of epigallocatechin gallate （EGCG） for the treatment of hepatic cirrhosis and its effects on telomerase activity. Methods The model of Wistar rats with hepatic cirrhosis was established by modified carbon tetrachloride （CC14） method; Wistar rats with CC14-induced cirrhosis were randomly divided into two groups： a model group treated with CC14, and a EGCG group treated with CC14 and EGCG by gavage. In addition, healthy rats were used as normal control. All rats were sacrificed after 8 weeks to collect serum samples for biochemical assays, hematoxylin and eosin （HE） staining and observation of histopathological changes in the structure of hepatic tissues under light microscopy were performed. Immunohistochemistry was used to detect telomerase reverse transcriptase （TERT） protein expression, and real-time polymerase chain reaction （real-time PCR） was performed to examine the mRNA expression of TERT in hepatic tissues. Results The untreated model rats showed typical hepatic cirrhosis histopathological changes. Compared with untreated model group,the degree of hepatic fibrosis was significantly relieved in the EGCG group. Serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） levels in EGCG group were significantly lower than that in untreated model group （P〈0.01）. Immunohistochemical results showed that the expression of TERT was increased in the untreated model group compared with the normal control group （P〈0.01）, while the expression of TERT in the EGCG group was lower than in the untreated model group （P〈0.05）. The mRNA expression of TERT was also lower in the EGCG groupthan in the untreated model group （P〈0.05）. Conclusion EGCG significantly inhibits the progression of hepatic fibrosis in rats, and effectively reduces the expression of TERT in hepatic tissues of rats with cirrhosis.

关 键 词：EGCG 肝硬化 TERT 大鼠 

分 类 号：R931.71[医药卫生—生药学]