叶状蔷薇珊瑚来源真菌Parengyodontium album SCSIO 40430次级代谢产物研究  

Chromanones from Montipora foliosa-derived Parengyodontium album SCSIO 40430

作　　者：黄艳冰 蒋晓东[1,2] 刘威 黄春帅 张丽萍[1] 诸晗宁 张长生[1] 张文军 HUANG Yan-Bing;JIANG Xiao-Dong;LIU Wei;HUANG Chun-Shuai;ZHANG Li-Ping;ZHU Han-Ning;ZHANG Chang-Sheng;ZHANG Wen-Jun(Key Laboratory of Tropical Marine Bio-resourses and Ecology,RNAMC Enter for Marine Microbiology Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,Guangdong 510301,China;University of the Chinese Academy of Sciences,Beijing 100049,China;State Key Laboratory of Applied Microbiology Southern China,South China Sea Resource Exploitation and Protection Collaborative lnnovation Center（SCS-REPIC）,SunYat-sen University,Guangzhou,Guangdong 510275,China)

机构地区：[1]中国科学院热带海洋生物资源与生态重点实验室中国科学院海洋微生物中心广东省海洋药物重点实验室中国科学院南海海洋研究所,广东广州510301 [2]中国科学院大学,北京100049 [3]省部共建华南应用微生物国家重点实验室南海资源开发和保护协同创新中心(SCS-REPIC)中山大学,广东广州510275

出　　处：《微生物学通报》2018年第9期1881-1888,共8页Microbiology China

基　　金：国家自然科学基金(31470172;41676165);中国科学院重点项目(QYZDJ-SSW-DQC004)~~

摘　　要：【背景】海洋来源真菌次级代谢产物由于具有化学结构新颖和生物活性多样的特点,是药物发现新的源泉之一。【目的】研究Parengyodontium album SCSIO 40430次级代谢产物及其抑菌活性。【方法】利用常压柱色谱、半制备HPLC等色谱学方法对P.albumSCSIO40430发酵液粗提物进行分离纯化;通过HR-ESI-MS、1H、13CNMR等光谱学方法,并结合文献数据,确定其产生的化合物结构;对分离得到的化合物进行抑菌活性测试。【结果】分离到3个苯并二氢吡喃酮类化合物,结构确定为Deoxyphomalone(1)、2-Ethyl-3,5-dihydroxy-11-methylchroman-9-one (2)、2-Ethyl-3-hydroxy-5-methoxy-11-methylchroman-9-one (3)。【结论】获得3个苯并二氢吡喃酮类化合物;活性测定结果显示化合物2对耐甲氧西林金黄色葡萄球菌(MRSAATCC43300)、苏云金芽孢杆菌(B.thuringiensisSCSIOBT01)有较好的生长抑制活性(MIC 16μg/mL)。[Background] Marine-derived fungi are prolific resources of natural products due to their diversity and ability to produce novel bioactive compounds. [Objective] To obtain the bioactive secondary metabolites from the fungus Parengyodontium album SCSIO 40430, which isolated from a Montipora foliosa sample from the South China Sea, and to evaluate for their antibacterial activity. [Methods] The compounds were isolated and purified by silica gel column, Sephadex LH-20 column and semi-prepartive HPLC chromatography. Their structures were identified by HR-ESI-MS, 1H, 13C NMR, and comparison with the data of literatures. [Results] Three compounds were isolated from the bmanone extract and structurely identified as deoxyphomalone （1）, 2-ethyl-3,5-dihydroxy-11-methylchroman- 9-one （2） and 2-ethyl-3-hydroxy-5-methoxy-11-methylchroman-9-one （3）. [Conclusion] Three chromanone compounds were aquired, and compounds 2 displayed antibacterial activities of Methicillin-resistant Stphylococcus and Bacillus thuringiensis SCSIO BT01 （MIC 16 μg/mL）.

关 键 词：Parengyodontium ALBUM SCSIO 40430 二氢吡喃酮 抗MRSA活性 

分 类 号：Q936[生物学—微生物学]