结缕草属植物种质资源遗传变异研究  

Genetic Variation Analysis of Zoysia willd. Accessions

作　　者：黄春琼[1] 刘国道[1] 白昌军[1] 唐军[1] 王文强[1] 丁西朋 刘一明 HUANG Chunqiong;LIU Guodao;BAI Changjun;TANG Jun;WANG Wenqiang;DING Xipeng;LIU Yiming(Tropical Crops Genetic Resources Institute,CATAS/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Danzhou,Hainan 571737,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室,海南儋州571737

出　　处：《热带作物学报》2018年第7期1259-1265,共7页Chinese Journal of Tropical Crops

基　　金：中国南方草地牧草资源调查(No.2017FY100601);中央级公益性科研院所基本科研业务费专项资金(No.1630032014028);现代农业产业技术体系建设专项(No.CARS-35);热带牧草种质资源保护项目;中国热带农业科学院热带作物品种资源研究所非营利性科研机构改革专项启动费项目(No.pzsfyl-201816)

摘　　要：利用SRAP标记技术研究45份结缕草属植物的遗传变异情况。从100对引物中筛选出13对扩增带型清晰、多态性较好的引物对其进行扩增,共扩增出113条条带,平均每对引物扩增出8.69条条带,多态性条带数为96条,多态性条带所占比例为84.16%,遗传相似性范围为0.54~0.96,平均为0.75。基于UPMAG(unweighted pair-group method with arithmetic means)法,在遗传相似性系数为0.74时,可将45份结缕草属种质分为4个类群,生态地理条件相似的种质优先聚在一起。本研究结果说明结缕草属植物种质间遗传变异比较广泛,为结缕草属植物种质资源的开发利用及新品种选育奠定了良好基础。Sequence-related amplifed polymorphism（SRAP） was used to detect the genetic variation and genetic ralationship among 45 Zoysia willd. accessions. A total of 13 primer pairs from the original 100 primer pairs were selected based on the polymorphic, clear and reproducible bands produced by them among 3 Zoysia accessions. A total of 113 bands were amplified by 13 SRAP primer pairs from 45 Zoysia accessions, and 96（84.16%） out of them were polymorphic. The genetic similarity coeffcients（GSC） among the 45 accessions ranged from 0.54 to 0.96 with an average of 0.75. Cluster analysis conducted by the unweighted pair-group method with arithmetic averages（UPGMA） separated the 45 Zoysia accessions into 4 distinct groups when the genetics similarity coefficient was 0.74. Results obtained clearly indicated significant variation existed in the wild Zoysia genotypes, which would provide a theoretical foundation for development and utilization of Zoysia germplasm resources and new variety breeding.

关 键 词：结缕草 遗传变异 SRAP 

分 类 号：S544.2[农业科学—作物学]