一株致多器官功能衰竭非O1/O139群霍乱弧菌的鉴定  

Identification of non-O1/non-O139 Vibrio cholerae causing multiple organ dysfunction syndrome

作　　者：陈昱希 黄健 陈安林[1,2] 陈泽慧 韩道宾[1] 骆诗露 徐梅[1] 李晓婷 闵迅 CHEN Yu-xi;HUANG Jian;CHEN An-lin;CHEN Ze-hui;HAN Dao-bin;LUO Shi-lu;XU Mei;LI Xiao-ting;MIN Xun(Department of Laboratory Medicine,Zunyi Medical College,Zu-nyi,Guizhou,563003,China;Department of Laboratory Medicine,The First Hospital Affiliated with Zunyi Medical College)

机构地区：[1]遵义医学院检验医学院,贵州遵义563003 [2]遵义医学院附属医院医学检验科

出　　处：《中国病原生物学杂志》2018年第8期831-834,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81460317);贵州省联合基金资助项目(No.黔科合LH字[2014]7553)

摘　　要：目的对1例不洁饮食致多器官衰竭患者血液中霍乱弧菌进行鉴定。方法取患者静脉血进行细菌的分离培养,观察细菌的形态特征,并进行生化试验、质谱鉴定、血清学检测、16SrRNA分析、毒力基因检测。结果分离菌初步鉴定为非O1/O139群霍乱弧菌。MALDI-TOF MS鉴定该菌为霍乱弧菌,置信度99.9%。16SrRNA扩增产物测序后经blast分析,与数据库中霍乱弧菌相似性为99%。该菌无毒力基因ctx、Tcp,毒力基因lolB、toxR、rtxC、hlyA、Hap、PrtV、ompU均阳性。结论该致病菌为非O1/O139群霍乱弧菌,患者发生急骤性多器官功能衰竭死亡可能与该菌携带lolB、toxR、rtxC、hlyA、Hap、PrtV、ompU等毒力基因密切相关。Objectives To identify a strain of Vibrio cholerae from the blood of a patient with multiple organ dysfunction syndrome caused by unclean food and water. Methods A strain was isolated from the venous blood of a patient and cultured,and its morphological characteristics were observed.The strain was subjected to biochemical testing,its characteristics were determined using matrix-assisted laser desorption/ionization mass spectrometry,serology was performed,and the 16 SrRNA sequences and virulence genes of the isolate were analyzed. Results This clinical isolate was confirmed to be non-O1/non-O139 V.cholerae.MALDI-TOF MS identified this strain as V.cholerae with a reliability of99.9%.16 SrRNA was amplified,and sequencing blast analysis indicated that the strain had 99% similarity to strains in the NCBI database.The virulence genes lolB,toxR,rtxC,hlyA,Hap,PrtV,and ompU were found in the strain while the virulence genes ctx and Tcp were not. Conclusion The pathogen was identified as non-O1/non-O139 V.cholerae,and the development of multiple organ dysfunction syndrome and death may be associated with virulence genes such as lolB,toxR,rtxC,hlyA,Hap,PrtV,and ompU.

关 键 词：霍乱弧菌 非O1/O139群 鉴定 毒力基因 

分 类 号：R378.3[医药卫生—病原生物学]