延边牛Y染色体遗传多样性与父系起源研究  

Study on Genetic DDiversity and Paternal Origin of Y Chromosome in Yanbian Cattle

作　　者：史红侠 姚一博 夏小婷 吕忠 党瑞华[2] 蓝贤勇[2] 陈宏[2] 雷初朝[2] SHI Hong-xia;YAO Yi-bo;XIA Xiao-ting;LV Zhonga;DANG Rui-hua;LAN Xian-yong;CHEN Hong;LEI Chu-zhao(Institute of Animal Health Supervision,Weinan,Shaanxi 714000;College of Animal Science and Technology,Northwest Agricultural ＆ Forestry University,Yangling,Shaanxi 712100;Yanbian Comprehensive Test Station of National Beef Cattle and Yale industrial Technology System,Yanbian,Jilin 13300)

机构地区：[1]渭南市动物卫生监督所,陕西渭南714000 [2]西北农林科技大学动物科技系统,陕西杨凌712100 [3]国家肉牛牦牛产业技术体系延边综合试验站,吉林延边133000

出　　处：《中国牛业科学》2018年第4期14-16,共3页China Cattle Science

基　　金：国家肉牛牦牛产业技术体系(CARS-37)资助

摘　　要：[目的]为了研究延边牛Y染色体遗传多样性以及父系起源。[方法]采用PCR扩增、直接测序和生物信息学方法,分析延边牛的2个Y-SNPs标记(UTY19和ZFY10)和2个Y-STRs标记(INRA189和BM861)的遗传多样性。[结果]Y-SNPs标记检测发现延边牛有Y1和Y2单倍型组,2个Y-STRs标记在延边牛品种检测发现只有INRA189表现多态性(98bp/102bp/104bp)。结合Y-SNPs与Y-STRs的分型结果,确定延边牛有3种Y染色体单倍型Y1-98-158、Y2-102-158和Y2-104-158,所占频率分别为0.031、0.938和0.031,其Y染色体单倍型多样度为0.1230±0.0777,表明延边牛父系遗传多样性很低,父系遗传稳定。[结论]延边牛为普通牛父系起源,其中Y2单倍型组占明显优势。[Objective]Exploring the Y chromosome genetic diversity and paternal origin of Yanbian cattle.[Methods]Genetic diversities of two Y-SNPs markers（UTY19 and ZFY10）and two Y-STRs markers（INRA189 and BM861）in Yanbian cattle were analyzed by PCR amplification,direct sequencing and bioinformatics analysis.[Results]Y-SNPs marker detection showed that Yanbian cattle had Y1 and Y2 haplotype groups,and two Y-STRs markers showed only SINR189 polymorphism（98 bp/102 bp/104 bp）in Yanbian cattle breeds.Combined with the typing results of Y-SNPs and Y-STRs,it was determined that Yanbian cattle had three Y chromosome haplotypes Y1-98-158,Y2-102-158 and Y2-104-158,which accounted for 0.031,0.938,and 0.031,respectively.Moreover,the haplotype diversity of the Y chromosome was 0.1230±0.0777,indicating that the genetic diversity of Yanbian cattle paternal is very low,and the paternal inheritance is stable.[Conclusion]Yanbian cattle was the origin of common cattle paternity,and the Y2 haplotype group had obvious advantages.

关 键 词：延边牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823[农业科学—畜牧学]