涠洲牛Y-SNPs和Y-STRs遗传多样性及父系起源研究  

Genetic Diversity and Paternal Origin of Y-SNPs and Y-STRs in Weizhou Cattle

作　　者：李秀良[1] 李芳玉 曹艳红[1] 吴柱月[1] 党瑞华[2] 李绍波[1] 陈宏[2] 雷初朝[2] LI Xiu-liang;LI Fang-yu;CAO Yan-hong;WU Zhu-yue;DANG Rui-hua;LI Shao-bo;CHEN Hong;LEI Chu-zhao(Animal Husbandry Institute of Guangxi Zhuang Autonomous Region,Nanning,Guangxi,530001,China;College of Animal Science and Technology,Northwest A＆F University,Yangling,Shaanxi 712100,China)

机构地区：[1]广西壮族自治区畜牧研究所,广西南宁530001 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国牛业科学》2018年第4期29-31,44,共4页China Cattle Science

基　　金：国家肉牛牦牛产业技术体系(CARS-37)资助;利用广西黄牛杂交培育优质雪花牛肉示范与推广(桂渔牧科201452035);广西良种肉牛繁育关键技术与产业化应用示范(桂科AA17204028)资助

摘　　要：[目的]为从分子水平上探究涠洲牛的遗传多样性、群体遗传结构及父系起源。[方法]本研究利用PCR测序及生物信息学方法,对28头涠洲公牛的2个Y-SNPs标记(UTY-19和ZFY-10)及2个Y-STRs标记(INRA189和BM861)进行多态性检测。[结果]发现28头涠洲牛全部为Y3单倍型组,根据Y-SNPs标记的核苷酸变异及Y-STRs标记的等位基因大小确定单倍型(Y-SNP-INRA189-BM861),结果显示28头涠洲牛有Y3-88-156和Y3-90-156两种单倍型,单倍型频率分别为89.29%和10.71%,说明涠洲牛只有1个瘤牛Y3父系起源。Y染色体单倍型多样度为0.1984±0.0924,表明涠洲牛的父系遗传多样性较低。[结论]涠洲牛属于瘤牛父系起源,遗传基础十分稳定。[Objective]This study was to explore the paternal genetic diversity,population genetic structure and origin of Weizhou cattle at the molecular level.[Method]The polymorphism of two Y-SNPs markers（UTY-19 and ZFY-10）and two Y-STRs markers（INRA189 and BM861）were detected in 28 Weizhou cattle using PCR sequencing and bioinformatics analysis.[Result]All tested Weizhou cattle belong to Y3 haplotype groups.Basing on the nucleotide variation of the Y-SNPs marker and the allele size of the Y-STRs marker,the haplotype（Y-SNP-INRA189-BM861）was determined.The results showed that28 Weizhou cattle had two haplotypes,Y3-88-156 and Y3-90-156,and the haplotype frequencies were 89.29% and 10.71%,respectively,indicating that only one zebu cattle Y3 paternal origin originated from Weizhou cattle.Moreover,the haplotype diversity of Y-chromosome was 0.1984±0.0924,indicating that Weizhou cattle had low genetic diversity.[Conclusion]Weizhou cattle belong to the zebu paternal origin with low paternal genetic diversity,indicating a very stable genetic basis.

关 键 词：涠洲牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823[农业科学—畜牧学]