渤海黑牛Y染色体遗传多样性及父系起源研究  

Genetic Diversity of Y Chromosome and Paternal Origins in Bohai Black Cattle

作　　者：常振华[1] 侯佳雯 宋恩亮[3] 成海建[3] 陈宏[2] 雷初朝[2] CHANG Zhen-hua;HOU Jia-wen;SONG En-liang;CHENG Hai-jian;CHEN Hong;LEI Chu-zhao(Weinan Vocational and Technical College,Weinan,Shaanxi 714000,China;College of Animal Science and Technology,Northwest A＆F University,Yangling,Shaanxi 712100,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100,China)

机构地区：[1]渭南职业技术学院,陕西渭南714000 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]山东省农业科学院畜牧兽医研究所,山东济南250100

出　　处：《中国牛业科学》2018年第4期41-44,共4页China Cattle Science

基　　金：国家肉牛牦牛产业技术体系(CARS-37)资助

摘　　要：[目的]为研究渤海黑牛的遗传多样性、群体遗传结构及父系起源。[方法]采用PCR扩增、琼脂糖凝胶电泳的方法,利用Y-SNPs和Y-STRs联合标记,对15头纯种渤海黑牛和15头与日本和牛杂交改良的渤海黑牛公牛进行遗传多样性检测。[结果]发现15头纯种渤海黑牛中普通牛Y1单倍型组所占频率为6.67%,普通牛Y2单倍型组所占频率为20.00%,瘤牛Y3单倍型组所占频率为73.33%,单倍型多样度为0.4476±0.1345;15头与日本和牛杂交改良的个体中,Y1单倍型组所占频率为40.00%,Y2单倍型组所占频率为26.67%,Y3单倍型组所占频率为33.33%,单倍型多样度为0.7048±0.0535。结果表明,渤海黑牛群体存在普通牛Y1、Y2和瘤牛Y3三种父系起源,遗传多样性较高。[结论]纯种渤海黑牛的父系以瘤牛为主,同时兼有普通牛Y2的种质特征。[Objective]This paper aims to study the genetic diversity,population genetic structure and paternal origin of Bohai black cattle.[Method]Y-SNPs and Y-STRs were analyzed in 15 pure bulls and 15 hybrid Bohai black cattle using direct DNA sequencing and microsatellite genotyping methods.[Result]The results indicated that the 15 pure individuals owned Y1 haplotype group with frequency of 6.67%,while frequency of Y2 haplotype group was20.00% and Y3 haplotype group with frequency of 73.33%.The 15 hybrid individuals owned Y1 haplotype group with frequency of 40.00%.Y2 haplotype group with frequency of 26.67% and Y3 haplotype group with frequency of 33.33%.In addition,the Y chromosome haplotype diversity of the pure and hybrid individuals was 0.4476±0.1345 and 0.7048±0.053,respectively.[Conclusion]The paternal line of purebred Bohai black cattle is mainly zebu cattle,and has the germplasm characteristics of Y2 in Bos taurus.

关 键 词：渤海黑牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823[农业科学—畜牧学]