径向展开薄层色谱法分离生物碱及其分离效能  

Separation of alkaloids using radial chromatography and its separation efficiency

作　　者：张梦婷 巩丹丹[1] 孙万阳[2] 孙国祥[1] ZHANG Mengting;GONG Dandan;SUN Wanyang;SUN Guoxiang(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;Institute of Traditional Chinese Medicine ＆ Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]暨南大学中药和天然药物研究所,广东广州510632

出　　处：《色谱》2018年第10期1045-1052,共8页Chinese Journal of Chromatography

基　　金：国家自然科学基金(90612002;81573586)~~

摘　　要：径向展开薄层色谱法是一种将样品由中心沿径向向外展开的简便、快速、高效的色谱方法。该文组装了简单的径向展开薄层色谱装置,并建立了朱砂安神丸的径向展开薄层色谱检测法,对其中的生物碱成分进行分离,研究了径向展开薄层色谱的分离特性。从薄层色谱基础理论出发,对径向展开薄层色谱和一般薄层色谱的分离效能进行了比对研究,设计试验进行计算和求解。证明了径向展开薄层色谱法更快、更高效、更经济,适用于生物碱等高极性样品分离。探索了径向展开薄层色谱法高分离效率的理论根源,这一研究思路也为理论创新提供了新的方法和思路。The radial thin layer chromatography （RTLC） is a chromatographic method for the simple, rapid and efficient separation of samples from the center along the radial direction. Simple device was assembled to separate the alkaloids contented in Zhusha Anshen Pills （ZSASP）. Based on the theory of thin layer chromatography, the test was designed to compare the separation efficiency of radial chromatography and general thin layer chromatography. The RTLC is faster, more efficient, more economical, and more suitable for the separation of high polar samples like alkaloids as proved. The theoretical root of the high separation efficiency of radial thin layer method was explored, which also provided new methods and ideas for theoretical innovation.

关 键 词：平面色谱 径向展开薄层色谱 生物碱 分离效能 试验设计验证 数据拟合 

分 类 号：O658[理学—分析化学]