HPLC测定黄精的指纹图谱  

Study on Chromatographic Fingerprint of Polygonati Rhizoma by HPLC

作　　者：李松涛[1] 于晓[1] 耿翠翠 刘志萍 车勇[1] 孔青[1] LI Song-tao;YU Xiao;GENG Cui-cui;LIU Zhi-ping;CHE Yong;KONG Qing(Shandong Medical Technician College,Taian 271000,China)

机构地区：[1]山东医药技师学院,山东泰安271000

出　　处：《食品与药品》2018年第5期336-339,共4页Food and Drug

基　　金：泰安市科技发展计划(201340629):泰山黄精产业化开发利用关键技术研究

摘　　要：目的建立山东泰安黄精的高效液相色谱指纹图谱。方法采用高效液相色谱法(HPLC)比较分析不同批次黄精的指纹图谱,并采用"中药色谱指纹图谱相似度评价系统(2004A版)"计算和处理测定的图谱。色谱条件:安捷伦Eclipse XDB-C18(150 mm×4.6 mm,5μm)色谱柱,乙腈-水溶液为流动相,梯度洗脱,流速1 ml/min,检测波长203 nm,柱温30℃。结果通过共有模式相似度分析可确定16个色谱峰,构成山东泰安黄精指纹图谱特征峰。利用该色谱条件比较山东泰安黄精和河北黄精的指纹图谱,二者有显著差异。结论HPLC建立的黄精指纹图谱稳定性、重复性较好,可为区分其他区域的黄精提供参考。Objective To establish HPLC fngerprint of Polygonati rhizoma in Taian. Methods Different batches of Polygonati rhizoma were analyzed by high performance liquid chromatography （HPLC）. The HPLC fngerprints were processed by the software of Chinese Medicine Chromatographic Fingerprints Similarity Evaluation System （version 2004A）. Agilent Eclipse XDB-C18 （150 mm×4.6 mm, 5 μm） column was used, and acetonitrile solution was used as mobile phase at a flow rate of 1 ml/min for the gradient elution. The detection wavelength was 203 nm. The analysis time was 65 min and the column temperature was 30 ℃. Results Under the selected chromatographic conditions, 16 chromatographic peaks were identified by common pattern similarity analysis, which constituted the characteristic peaks of the fngerprint of Polygonati rhizoma in Taian. Obvious difference were found between the fngerprints of Polygonati rhizoma in Taian and Hebei. Conclusion The fngerprint of Polygonati rhizoma set up by HPLC is stable and repeatable, and can provide the reference to distinguish Polygonati rhizoma in Taian and other areas.

关 键 词：黄精 高效液相色谱 指纹图谱 

分 类 号：R284[医药卫生—中药学]