脊髓组织存放时间对冰冻组织切片免疫荧光的影响  

Effect of Storage Time of Spinal Cord Tissue on Immunofluorescence of Frozen Tissue Sections

作　　者：何祥[1] 胡悦 蒋玲 刘树春[1] HE Xiang;HU Yue;JIANG Ling;LIU Shuchun(Department of Anesthesiology,Guizhou People＇s Hospital,Guiyang 550002,Guizhou,China;Department of Anesthesiology,the First People＇s Hospital of Shuangliu,Chengdu 610200,Sichuan,China)

机构地区：[1]贵州省人民医院麻醉科,贵州贵阳550002 [2]成都市双流区第一人民医院麻醉科,四川成都610200

出　　处：《贵州医科大学学报》2018年第9期1007-1011,共5页Journal of Guizhou Medical University

基　　金：贵州省卫计委科学技术基金项目(gzwjkj2018-1-018)

摘　　要：目的:研究脊髓组织在4%多聚甲醛固定液中的存放时间对冰冻切片抗原NeuN表达的影响。方法:固定于4%多聚甲醛中1年、3月、2月、1月、2周、1周及24 h的脊髓组织,在相同的条件下制作冰冻切片,通过对神经元核抗原(NeuN)的免疫荧光的检测,观察脊髓组织标本在4%多聚甲醛中的固定时间对NeuN表达的影响。结果:在脊髓前角NeuN的表达中,组织固定1月、2月、3月及1年的NeuN阳性率与新鲜标本及组织固定1周、2周比较呈现显著性下降(P <0. 05);在脊髓后角NeuN的表达中,组织固定2月、3月及1年的NeuN阳性率与新鲜标本相比呈现显著性下降(P <0. 05),同时组织固定1年的NeuN阳性率与组织固定1周相比也呈现显著性下降(P <0. 05);在脊髓中央管NeuN的表达中,组织固定3月、1年的NeuN阳性率与新鲜标本及组织固定1周相比呈现显著性降低(P <0. 05),在脊髓组织整体NeuN的表达中,组织固定3月及1年的NeuN阳性率与新鲜标本及组织固定1周、2周比较呈现显著性下降(P <0. 05),同时组织固定2月与新鲜组织及组织固定1周比较呈现显著性下降(P <0. 05)。结论:在同样的环境和条件下,冰冻切片的组织标本越新鲜,其抗原的表达越强。Objective：To study the effect of storage time of spinal cord tissue in 4% paraformaldehyde fixation solution on the expression of antigens NeuN in frozen sections. Methods： Spinal cord tissues fixed at 4% paraformaldehyde for 1 year, 3 months, 2 months, 1 week and 24 h were made into frozen sections under the same conditions. The effect of fixed time of spinal cord tissue in 4% paraformaldehyde on the expression of （NeuN） was observed by immunofluorescence detection of neuronal nuclear antigen （NeuN）. Results： In the expression of NeuN of the anterior horn of spinal cord, the positive rate of NeuN in tissue fixed for 1 month, 2 months, 3 months and 1 year was significantly lower than that of fresh specimen and tissue fixation for 1 week and 2 weeks（ P 〈0.05）. In the expression of NeuN of the posterior horn of spinal cord, the positive rate of NeuN in tissue fixed for 2 months, 3 months and 1 year was significantly lower than that of fresh specimens（ P 〈0.05）. At the same time, the positive rate of NeuN in 1 year of tissue fixation was significantly lower than that of 1 week of tissue fixation （P 〈0.05）. In the expression of NeuN of the central canal of spinal cord, the positive rate of NeuN in tissue fixed for 3 months and 1 year was significantly lower than that in fresh specimens and tissue fixation for 1 week（ P〈 0.05）. In the expression of NeuN of spinal cord tissue as a whole, the positive rate of NeuN in tissue fixed for 3 months and 1 year was significantly lower than that in fresh specimen and tissue fixation for 1 week and 2 weeks（ P 〈0.05）. At the same time, two months of tissue fixation showed a significant decrease compared with fresh tissue and tissue fixation for 1 week（ P 〈0.05）. Conclusion： Under the same environment and conditions, the fresher the frozen sections are, the stronger the antigen expression is.

关 键 词：冰冻切片术 免疫荧光测定 脊髓 组织 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R34[医药卫生—基础医学]