高糖对小鼠肾小球内皮细胞中的缺氧诱导因子-1α及血管内皮生长因子表达的影响  

Effect of high glucose on expression of HIF-1α and VEGF in mouse glomerular endothelial cells

作　　者：余玲[1] 董瑞鸿[1] 宋秋艳[1] 甄月巧[1] 赵明明 桑艳红[1] 郭珏涵 Yu Ling;Dong Ruihong;Song Qiuyan(The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 45005)

机构地区：[1]郑州大学第五附属医院内分泌科,河南郑州450052

出　　处：《中国现代医药杂志》2018年第9期1-5,共5页Modern Medicine Journal of China

基　　金：河南省医学科技攻关计划项目(编号:201102007)

摘　　要：目的观察高糖及高糖联合缺氧对小鼠肾小球内皮细胞(glomerular endothelial cells)中的缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法体外培养肾小球内皮细胞,将细胞随机分为四组:正常对照组、高糖非缺氧组(不同血糖浓度)、缺氧对照组以及高糖缺氧组(不同血糖浓度),将细胞培养72h后采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)及逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)检测这四组肾小球内皮细胞中HIF-1α、VEGF的浓度及m RNA表达水平的变化。结果高糖非缺氧组肾小球内皮细胞中HIF-1α,VEGF的浓度及m RNA的表达水平与正常组比较有统计学差异(P<0.05);缺氧对照组肾小球内皮细胞中HIF-1α,VEGF的浓度及m RNA的表达水平显著高于正常组与高糖非缺氧组(P<0.05);高糖缺氧组肾小球内皮细胞中HIF-1α,VEGF的浓度及m RNA的表达水平显著高于其它三组(P<0.05),HIF-1α,VEGF浓度及m RNA的表达水平随着血糖浓度的升高而逐渐升高。结论 HIF-1α及VEGF与糖尿病肾病的发展密切相关,在糖尿病肾病治疗的早期,通过血糖的控制能够抑制HIF-1α及VEGF水平的增加,有效地保护内皮细胞,稳定肾脏功能,减缓糖尿病肾病的进展。Objective To observe the effect of high glucose and high glucose combined with hypoxia on HIF-1α and VEGF in mouse glomerular endothelial cells. Methods Mouse glomerular endothelial cells were randomly divided into four groups： normal control group, high glucose group, hypoxia control group and hypoxia combined with high glucose group. After 72 hours, the expression of HIF-1α, VEGF and mRNA levels were detected by reverse transtription polymerase chain reaction （RT-PCR） and ELISA. Results There were significant differences on the concentration and mRNA expression of HIF-1α, VEGF and mRNA between normal group and high glucose group（P〈0.05）. The concentration and mRNA expression of HIF-1α, VEGF and mRNA of hypoxia control group was significantly higher than normal control group and high glucose group（P〈0.05）; The concentration and mRNA expression of HIF-1α, VEGF and mRNA of hypoxia combined with high glucose group was significantly higher than other three groups（P〈0.05）, the concentration and mRNA expression of HIF-1α and VEGF was increased with the rising of blood glucose concentration. Conclusion HIF-1α and VEGF are closely related to the development of diabetic nephropathy. In the early stage of diabetic nephropathy, the control of blood glucose can inhibit the increase of HIF-1α and VEGF, protect endothelial cells effectively, stabilize renal function and slow down diabetic nephropathy progress.

关 键 词：高糖 缺氧 小鼠肾小球内皮细胞 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]