牛心朴子草抗植物病毒组分的生物活性研究  

STUDIES ON BIOACTIVITIES OF CYNANCHUM KOMAROVII AL.AS INHIBITER AGAINST PLANT VIRUSES

作　　者：姚宇澄[1] 杨火召 高俊[1] 安天英[2] 于学舜[1] 李广仁[2] 黄润秋[2] 

机构地区：[1]内蒙古工业大学化工学院,内蒙古呼和浩特010062 [2]南开大学元素有机化学研究所,天津300071

出　　处：《内蒙古工业大学学报（自然科学版）》2002年第1期1-4,共4页Journal of Inner Mongolia University of Technology：Natural Science Edition

基　　金：国家自然科学基金 No.2 9862 0 0 3;内蒙古自然科学基金 No.980 2 0 3资助项目

摘　　要：牛心朴子草地上部分醇浸粗提物 (H)对烟草花叶病毒 (TMV)有高抑制活性 .该草抗植物病毒有效组分是菲并吲哚里西定生物总碱 (G) .半叶枯斑法测定(G)制剂对 TMV、马铃薯 Y病毒 (PVY)、和芜菁花叶病毒 (TUMV)呈现高活性 ;用酶联免疫法 (Elisa)测定 (G)制剂对 TUMV的最佳浓度防效 ,与枯斑法测定结果基本相符 .(G)中含量最丰的 (1 ) 7-脱甲氧基娃儿藤碱 (antofine)是该草活性最高的主组分 ,它在 1 0 - 6 g/ ml浓度的 TMV抑制率达 60 % .Cynanchum Komarovii AL.Lijinski (Asclepiadaceae) is a species of wild shrub,widely distributed in the northeast part of the People′s Republic of China.The alcoholic extract from the aerial parts of C.Komarovii demonstrates potent antiviral activity against tobacco mosaic virus (TMV),potato virus Y(PVY) and turnip mosaic virus (TUMV).A bioassay directed fractionation approach has been adopted and phenanthroindolizidine alkaloids (G),including two known compounds,7 demethoxytylophorine (antofine) (1) and N oxide 7 demethexytylophorine (N oxide antofine) (2),have been isolated from the extract.Bioassay performed by the conventional half leaf method has revealed that the alkaloids (G) show high inhibitory activity against TMV,PVY and TUMV.The major active alkaloid (1) exhibits 60% inhibition against TMV at a concentration of 1.0×10 -6 g/ml,while a commercial antivirus DHT only exhibits 50% inhibition at 5×10 4 g/ml under the same conditions.

关 键 词：抗植物病毒组分 生物活性 牛心朴子草 植物病毒抑制剂 菲并吲哚里西定碱 7-脱甲氧基娃儿藤碱 农药 

分 类 号：S482[农业科学—农药学]