毒死蜱对斑马鱼的急性毒性试验  

The acute toxicity test of chlorpyrifos to zebra fish

作　　者：霍奕安 胡秀彩 吕爱军[1] 孙敬锋 Huo Yian;Hu Xiucai;Lv Aijun;Sun Jingfeng(Tianjin Key Lab of Aqua-Ecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;Lab of Innovation and entrepreneurship,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院水产学院天津市水产生态及养殖重点试验室,天津300384 [2]天津农学院创新创业试验室,天津300384

出　　处：《水产养殖》2018年第10期34-36,41,共4页Journal of Aquaculture

基　　金：国家大学生创新训练计划项目(201810061036);天津农学院大学生创新训练计划项目(201810061174);天津农学院十三五综投平台项目(J01009030638);天津市自然科学基金项目(16JCZDJC33500;18JCYBJC299000);天津市水产产业技术体系创新团队(ITTFRS2017009);天津市高等学校"创新团队培养计划"现代水产生态健康养殖(TD13-5089)

摘　　要：为了研究毒死蜱对于斑马鱼的毒性和安全评价进行急性毒性试验,以24～96 h半致死浓度(LC50)来判定斑马鱼对毒死蜱的敏感性。结果表明,毒死蜱24 h LC_(50)、48 h LC_(50)、72 h LC_(50)、96 h LC_(50)分别为1.345 mg/L、1.080 mg/L、0.992 mg/L、0.926mg/L,安全质量浓度(SC)为0.209 mg/L。参照我国化学物质对鱼类毒性分级标准,判定毒死蜱对斑马鱼急性毒性为Ⅰ级,根据《化学农药环境安全评价试验准则》中农药对鱼类的毒性等级划分,可以判定毒死蜱对斑马鱼为高毒。To study the toxicity and safety evaluation of chlorpyrifos pesticides for zebrafish, the method of acute toxicity test in 24-96 h half lethal concentration （LC50） was used to determine the sensitivity of zebrafish to chlorpyrifos. Experimental results showed that 24 h LC50, 48 h LC50, 72h LC50 and 96 h LC50 of chlorpyrifos was 1.3454 mg/L, 1.080 mg/L, 0.992 mg/L and 0.926 mg/L, respectively; and safe mass concentration （SC） was 0.209 mg/L. With reference to our country chemical classification standard toxicity to fish, acute toxicity of chlorpyrifos for zebra fish was determine as grade Ⅰ . According to the chemical pesticide environmental safety evaluation test guidelines pesticide toxicity to fish in the hierarchy, chlorpyrifos can be determined as high toxic for zebrafish.

关 键 词：斑马鱼 急性毒性 毒死蜱 半致死浓度 安全质量浓度 

分 类 号：S482[农业科学—农药学]