体外血管内皮细胞培养装置中弹性基底的应力研究  

Stress Analysis on Elastic Substrate of in vitro Endothelial Cell Culture Device

作　　者：张迎 丁皓[1,2] 杨乾 雷亚楠[1] 兰海莲[1] ZHANG Ying;DING Hao;YANG Qian;LEI Yanan;LAN Hailian(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai University of Medicine ＆ Health Sciences,Shanghai 20]3]8,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]上海健康医学院医疗器械学院,上海201318

出　　处：《医用生物力学》2018年第4期354-359,共6页Journal of Medical Biomechanics

基　　金：上海市自然科学基金项目(17ZR1413500)

摘　　要：目的对一种体外血管内皮细胞动态培养装置中弹性基底的应力情况进行研究,使装置更接近人体血流动力学环境,实验并观察不同张应力情况下平行平板流动腔中弹性基底切应力的变化。方法采用一系列静态拉伸试验拟合动态拉伸的情况,即将两种不同厚度硅胶片置于装置中,从0%开始,分别以每次间隔10%拉伸率做静态拉伸（0%、10%、20%、30%）,并在保持其拉伸率不变的情况下,计算硅胶片拉伸后的腔体高度。通过计算切应力得到不同拉伸率下的切应力曲线,分析比较切应力随硅胶片厚度变化的规律。结果实验结果与理论分析基本一致。当拉伸率为30%时,0.5 mm厚度硅胶片随拉伸率的变化（腔体高度的变化）,对平行平板流动腔中切应力会产生一定影响,平均和最大切应力分别减小10.1%和10.4%。结论计算平行平板流动腔中切应力必须考虑引入张应力后弹性基底厚度变化所引起的影响因素。研究结果可为体外血管内皮细胞的培养以及设计研制新型平行平板流动腔提供实验技术。Objective To study the stress on elastic substrate of an in vitro endothelial cell dynamic culture device,whose hemodynamic environment is designed to simulate the human body,and to test and observe the shear stress changes in elastic substrate of parallel plate flow chamber under different tensile stresses. Methods A series of static tensile tests were adopted to fit the condition of dynamic stretching. Namely,the silicone sheet with 2 different thicknesses were put into the device,and then applied with static stretch at the interval of 10% tensile rate （ 0%,10%,20%,30%）,and under the condition of maintaining its tensile rate,the chamber height after the stretch of silicon sheet was calculated. Based on the calculation of shear stress,shear stress curves at different tensile rates were obtained,to make comparative analysis on variation of the shear stress with the thickness of silicon sheet. Results The experimental result was consistent with the theoretical analysis. When the tensile rate was 30%,silicon sheet with 0.5mm thickness would produce certain influence on shear stress of parallel plate flow chamber along with the change of tensile rate（ the height of chamber）,and the average and maximum shear stress were reduced by 10.1% and 10.4%,respectively. Conclusions The influence factors caused by the change of elastic substrate thickness after the introduction of tensile stress must be taken into account for the calculation of shear stress in parallel plate flow chamber. The result can provide experimental technology for the culture of endothelial cells in vitro and the design and development of novel parallel plate flow chamber.

关 键 词：动态培养装置 弹性基底 应力分析 血液动力学 

分 类 号：R318.01[医药卫生—生物医学工程]