高效液相色谱法快速筛查环境水体中16种氟喹诺酮类抗生素及质谱确证  

Rapid determination of 16 fluoroquinolones in environmental water by high performance liquid chromatography and their verification by mass spectrometry

作　　者：李玉静[1,2] 陈志冉 王雪平[1,2] LI Yu-jing;CHEN Zhi-ran;WANG Xue-ping(Yellow River Conservancy Technical Institute, Kaifeng 475004, China;Key Laboratory of Green Coating Materials of Kaifeng City, Kaifeng 475004, China)

机构地区：[1]黄河水利职业技术学院,河南开封475004 [2]开封市绿色涂层材料重点实验室,河南开封475004

出　　处：《现代化工》2018年第9期237-241,共5页Modern Chemical Industry

基　　金：河南省科技厅支持项目(172102410035);黄河水利职业技术学院2016年科技项目(2016KXJS009)

摘　　要：利用HLB固相萃取柱对环境水体中16种氟喹诺酮类抗生素(FQs)进行富集、净化,经可变波长扫描紫外检测器测定,建立了高效液相色谱法快速筛查环境水体中残留FQs种类及其残留量;对于疑似FQs残留阳性水样,采用液相色谱-串联质谱法进行进一步确证。结果表明,16种FQs在5.0~100.0μg/mL范围内,FQs质量浓度与其峰面积具有良好的线性关系,相关系数为0.998 9~0.999 9,检出限为0.9~7.3μg/mL,定量限为3.0~24.3μg/mL,平均回收率为89.3%~98.6%,相对标准偏差为0.9%~3.1%。HLB solid-phase extraction column is used to enrich and purify 16 kinds of fluoroquinolones（FQs） in environmental waters.After detection by a variable wavelength scanning ultraviolet detector,the method for determination of remindering FQs in environmental water by high performance liquid chromatography are established. Suspected FQs residual positive water samples are confirmed by liquid chromatography-mass spectrometry.It is found that in the range of5. 0-100. 0 μg/mL,the concentrations of 16 kinds of FQs all have a good linear relationship with their peak areas,with correlation coefficient ranging from 0. 998 9 to 0. 999 9,detection limit ranging from 0. 9 μg/mL to 7. 3 μg/mL,quantitation limit ranging from 3. 0 μg/mL to 24. 3 μg/mL,average recoveries ranging from 89. 3% to 98. 6% and the relative standard deviations ranging from 0. 9% to 3. 1%.

关 键 词：氟喹诺酮类抗生素 高效液相色谱 质谱确证 环境水体 

分 类 号：TQ460.9[化学工程—制药化工]