谷氨酰胺对产生内质网应激反应的A549细胞JNK,pJNK,CHOP,GRP78表达水平的影响  

Effects of glutamine on the expressions of JNK,pJNK,CHOP and GRP78 in A549 cells that produce endoplasmic reticulum stress

作　　者：李红子[1] 金正勇[1] 金梅花[1] 张有辰 LI Hongzi;JIN Zhengyong;JIN Meihua;ZHANG Youchen(Department of Pediatric,Affiliated Hospital of Yanbian Univeisity,Yanji 133000,Jilin,China)

机构地区：[1]延边大学附属医院儿科,吉林延吉133000

出　　处：《延边大学医学学报》2018年第2期91-93,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]探讨谷氨酰胺对产生内质网应激反应的A549细胞的保护作用.[方法]取A549细胞进行培养,分为5个组,其中空白对照组培养液中只有A549细胞,衣霉素组培养液中加入A549细胞和衣霉素1mg/L,GLN4组培养液中加入A549细胞、衣霉素1mg/L和4mmol/L谷氨酰胺,GLN8组培养液中加入A549细胞、衣霉素1mg/L和8mmol/L谷氨酰胺,GLN12组培养液中加入A549细胞、衣霉素1mg/L和12mmol/L谷氨酰胺.利用Western blot方法检测各组细胞培养12h后的JNK,pJNK,CHOP及GRP78表达水平.[结果]与空白对照组比较,衣霉素组pJNK,CHOP及GRP78表达水平均明显升高(P<0.001),而JNK表达水平未见明显改变(P>0.05),提示衣霉素诱导A549细胞产生了内质网应激.与衣霉素组比较,GLN4,GLN8及GLN12组pJNK,CHOP,GRP78表达水平均明显降低(P<0.05).[结论]谷氨酰胺对产生内质网应激的A549细胞具有保护作用.OBJECTIVE To explore the protective effects of glutamine on the A549 cells that produce endoplasmic reticulum stress.METHODS A549 Cells were cultured and divided into 5 groups：blank control group（only A549 cells in the culture medium）,tunicamycin group（cultured A549 cells with 1 mg/L tunicamycin in the culture medium）,GLN4 group（cultured A549 with 1 mg/L of tunicamycin and 4 mmol/L glutamine）,GLN8 group（cultured A549 cells with 1 mg/L of tunicamycin and 8 mmol/L glutamine）,GLN12 group（cultured A549 cells with 1 mg/L tunicamycin and 12 mmol/L glutamine）.Western blot was used to detect the expressions of pJNK,JNK,CHOP and GRP78 in A549 cells of each group after 12 hours’ culture.RESULTS The expression levels of pJNK,CHOP and GRP78 were increased significantly in tunicamycin group compared with the control group（P 〈0.001）,while the expression level of JNK did not change significantly（P〉0.05）,which indicated tunicamycin could induce A549 cells to produce the endoplasmic reticulum stress.As compared with the tunicamycin group,the expressions of pJNK,CHOP and GRP78 were decreased in all glutamine plus tunicamycin groups（P〈0.05）.CONCLUSION Glutamine had protective effects on A549 cells that produce endoplasmic reticulum stress.

关 键 词：内质网应激 A549细胞 谷氨酰胺 JNK pJNK CHOP/GADD153 GRP78/Bip 衣霉素 

分 类 号：R96[医药卫生—药理学]