植物中phasiRNA研究进展  

Recent studies on phasiRNAs in plants

作　　者：朱成新 熊伟 莫蓓莘 ZHU Cheng-Xin;XIONG Wei;MO Bei-Xin(Plant Epigenetic Laboratory,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]深圳大学生命与海洋科学学院植物表观遗传实验室,深圳518060

出　　处：《生命科学》2018年第8期831-839,共9页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金项目(31571332)

摘　　要：植物phasiRNA(phased,secondary,small interfering RNA)是一类内源性的、起调控作用的非编码小RNA(sRNA),它在植物的生长、发育、生殖以及抗病中发挥着重要作用。PhasiRNA初级转录本由Pol Ⅱ(RNA polymerase II)转录,随后miRNA(microRNA)引导AGO蛋白对phasi RNA初级转录本上的靶位点进行剪切,剪切后的phasiRNA前体片段(剪切位点的上游或下游片段)继而被RDR6(RNA-dependent RNA polymerases 6)复制成双链RNA,双链RNA被DCL4切割成一系列首尾相接,具有一定相位的21-nt或24-nt的小RNA,即phasiRNA。成熟的phasiRNA与AGO蛋白组装成沉默复合体,作用于靶基因,发挥转录后调控的功能。该文对植物phasiRNA的生物合成通路、生物学功能及其进化进行综述。Plant phasiRNAs reproduction（phased,well secondary,resistance small virus.interfering The RNAs）play important roles in plant are growth,development,by as as to primary phasiRNAs（pri-phasiRNAs）transcripted Pol Ⅱ（DNA dependant form RNA polymerase Ⅱ）.Then,pri-phasiRNAs are cleaved by AGO protein,which RNA is guided by miRNA,to of with the precursor phasiRNAs（pre-phasiRNAs）.One RDR6 portion of single-strand（upstream or downstream the miRNA cleavage site）will be converted into by（RNA-dependent RNA polymerases 6）into double-strand assembles RNA,which is then into the cut by DCL4 21-nt to or 24-nt phased target mature phasiRNAs.Mature phasiRNA AGO protein review silencing biosynthesis complex influence biological gene expression evolution at the posttranscriptional level.This paper will pathway,function and of phasiRNA in plants.

关 键 词：非编码小RNA phasiRNA ta-siRNA 转录后调控 

分 类 号：Q752[生物学—分子生物学] Q946