人胚胎干细胞建系的研究进展  

Progress on derivation of human embryonic stem cell

作　　者：陈枕枕 牛昱宇[1] CHEN Zhen-Zhen;NIU Yu-Yu(College of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500

出　　处：《生命科学》2018年第8期906-910,共5页Chinese Bulletin of Life Sciences

基　　金：国家重点基础研究发展计划项目(2016-YFA0101401)

摘　　要：人胚胎干细胞(human embryonic stem cells,h ESCs)具有自我更新和分化的潜能,自成功建立细胞系以来学者们一直在改进建系方法和培养体系,如避免异源污染(基质胶的研究、无饲养层和无血清培养体系的研究等)以建立优质的细胞系,获得更高全能性的干细胞(采用不同小分子的组合培养干细胞或建系),这些研究已经取得巨大成果。现通过胚胎来源、内细胞团(inner cell mass,ICM)分离、h ESCs培养体系3个方面综述h ESCs建系的研究进展。Human embryonic stem cells（h ESCs） have the potential of self-renewal and differentiation. Since it has been successfully established from embryo, scholars have been improving the method and culture system to obtain high-quality cell lines. The process of derivation is especially important cause it＇s the basis for follow-up experiments. This review summarizes embryonic origin, inner cell mass（ICM） separation and h ESC culture system to present the progress of h ESC lineage.

关 键 词：人 胚胎干细胞 建系 细胞培养法 

分 类 号：Q813[生物学—生物工程] R329[医药卫生—人体解剖和组织胚胎学]