小鼠特应性皮炎建模方法的改进  

Establishment of Atopic Dermatitis Mouse Model

作　　者：纪莲[1] 马铁[2] 狄正鸿[3] 刘冬妍[1] JI Lian;MA Tie;DI Zheng-hong;Liu Dong-yan(Department of Medical Research Center;Department of Pathology Department;Department of Dermatology of Shengjing Hospital,China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院实验研究中心,沈阳110004 [2]中国医科大学附属盛京医院病理科,沈阳110004 [3]中国医科大学附属盛京医院皮肤科,沈阳110004

出　　处：《实验动物与比较医学》2018年第4期267-271,共5页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(81301366)

摘　　要：目的用卵清蛋白(OVA)致敏小鼠特应性皮炎(atopic dermatitis,AD),建立一种更为简便、稳定的小鼠AD模型。方法采用6周龄BALB/c雌性小鼠,第1日腹腔注射200μg/m L OVA溶液0.5 m L系统致敏,第6日皮下注射100μg/m L OVA溶液0.5 m L再次致敏。并于第18、39日背部局部贴含50μL,1 000μg/m LOVA的1.5 cm2的无菌纱布,每日更换,分2次局部致敏,各持续1周。第46日,观察小鼠皮肤及其病理学改变,酶联免疫吸附法(ELISA)测定血清中总Ig E水平,统计成模率及动物死亡情况。结果 AD模型小鼠皮肤肉眼可见潮红、脱屑、偶见结痂等皮炎改变;病理可见表皮细胞增厚,过度角化,真皮内淋巴细胞等浸润。模型组小鼠血清总Ig E水平为(6 296.17±1 021.28)ng/L,显著高于空白对照组的(1 280.97±501.24)ng/L和生理盐水组的(1 327.57±629.71)ng/L(均P<0.01)。成模率为94%,死亡率6%。结论该方法可以成功建立小鼠AD模型,造模方法简单,成模率高。Objective To explore a novel method for atopic dermatitis（AD） model in mouse induced by ovalbumin（OVA）. Method The 6-weeks BALB/c female mice were subcutaneously injected with 0.5 m L of 200 μg/m L OVA solution on 1 st day and subcutaneously injected with 0.5 m L of 100 μg/m L OVA solution on 6 th day to induce systemic sensitization again. On 18 th day and 39 th day, 1.5 cm2 sterile gauze with 50 μL, 1 000 μg/m L OVA solution were attached on the back of the mice and replaced every day for consecutive 7 days for the second local sensitization. On 46 th day, the pathological changes in mouse skins were observed to judge whether mouse AD model was successfully established and the rates of being model and the mortality rate was calculated. Result The dermatitis changes, such as scab, flushing, desquamation, and so on, were shown in AD model mice. Also, the epidermal cells were thickened and the dermis was infiltrated with lymphocytes in AD model mice. The total serum Ig E level in the AD model mice [（6 296.17±1 021.28） ng/L] significantly increased as compared with blank control mice [（1 280.97 ± 501.24） ng/L]（P〈0.01） and the mice injected with saline [（1 327.57 ±629.71） ng/L]（P〈0.01）. The rates of AD model and the mortality were respectively 94% and 6%.Conclusion Our study introduces a simple and stable method for mouse AD model induced by OVA.

关 键 词：小鼠模型 特异性皮炎(AD) 卵清蛋白(OVA) 

分 类 号：Q95-33[生物学—动物学]