子宫内膜异位症及卵巢癌患者血清脑源性神经营养因子水平的表达及意义  

Expression and significance of serum BDNF in patients with endometriosis and ovarian cancer

作　　者：吴亚男 李红霞[1] 孟宪宁 王宏利[1] 魏玲[1] 霍志平[1] WU Ya-nan;L;MENG Xian-ning;WANG Hong-li;WEI Ling;HUO Zhi-ping(Department of Obstetrics and Gynecology,China National Petroleum Corporation Central Hospital,Langfang Heibei 065000,P.R.China)

机构地区：[1]中国石油天然气集团公司中心医院妇产科,河北廊坊065000

出　　处：《中国计划生育和妇产科》2018年第9期32-34,38,共4页Chinese Journal of Family Planning & Gynecotokology

基　　金：河北省廊坊市科学技术局科研课题(项目编号:2017013145)

摘　　要：目的本研究旨在探讨子宫内膜异位症(endometriosis,EMs)及卵巢癌患者血清中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)水平的表达及意义。方法收集2014年5月至2017年8月中国石油天然气集团公司中心医院收治的EMs患者82例(内异症组)和卵巢癌患者96例(卵巢癌组),以及同期来院体检的健康女性50例(对照组)。应用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)方法检测患者血清中BDNF的表达水平,比较3组间的差异。比较按照美国生育学会(American Fertility Society,AFS)不同分期的EMs患者血清BDNF水平的表达差异,并分析卵巢癌患者血清BDNF水平与临床病理特征之间的关系。结果术前内异症组与卵巢癌组的血清BDNF水平均明显高于对照组,而卵巢癌患者的血清BDNF水平又明显高于内异症组;术后内异症组与卵巢癌组的血清BDNF水平均较术前明显降低,但仍高于对照组,差异均有统计学意义(P<0.05);术后卵巢癌患者的血清BDNF水平与内异症组患者比较差异无统计学意义(P>0.05)。不同AFS分期的内异症患者的血清BDNF水平比较,Ⅰ~Ⅱ期患者明显低于Ⅲ~Ⅳ期(P<0.05);出现淋巴结转移的卵巢癌患者血清BDNF水平与无转移患者间比较,差异无统计学意义(P>0.05);浆液型肿瘤患者血清BDNF水平明显高于黏液型肿瘤、透明细胞癌和子宫内膜样腺癌(P<0.05),但黏液型肿瘤、透明细胞癌和子宫内膜样腺癌的血清BDNF水平比较差异无统计学意义(P>0.05);临床分期Ⅰ~Ⅱ期患者的血清BDNF水平明显低于Ⅲ~Ⅳ期,差异有统计学意义(P<0.05)。结论 BDNF在卵巢癌与EMs患者的血清中均呈高表达,但卵巢癌患者的表达更高。血清BDNF水平在不同AFS分期的EMs患者间存在差异,也与卵巢癌患者的组织学类型和临床分期有关。血清BNDF蛋白在卵巢癌与EMs的鉴别方面具有一定意义。Objective This study aimed to investigate the expression and significance of serum brain-derived neurotrophic factor（ BDNF） levels in patients with endometriosis（ EMs） and ovarian cancer. Methods 82 patients with EMs collected in China National Petroleum Corporation Central Hospital from May 2014 to August 2017 were set up as EMs group and 96 cases of ovarian cancer as ovarian cancer group. 50 healthy women（ normal endomtrium） were collected as control group at the same time. Enzyme-linked immunosorbent assay（ ELISA） was used to detect the expression level of BDNF in the serum of patients and the differences of the three groups were compared. To compare the differences of serum BDNF levels in EMs patients with different AFS stages and analyze the relationship between the serum level of BDNF and clinicopathological features in patients with ovarian cancer. Results The levels of serum BDNF in preoperative EMs group and ovarian cancer group were significantly higher than those in control group,while the levels of serum BDNF in ovarian cancer patients were significantly higher than those in EMs group. The serum levels of BDNF in patients with EMs and ovarian cancer were significantly improved compared with those before operation,but still higher than those in control group,the differences were statistically significant（ P〈0. 05）. However,the serum BDNF level in patients with ovarian cancer was not significantly different from that in patients with EMs group（ P〈0. 05）. The difference of serum BDNF levels in patients with EMs with different stages of AFS was compared,the patients in stage Ⅰ to Ⅱ were significantly lower than stage Ⅲ to Ⅳ（ P〈0. 05）.There was no significant difference in serum BDNF between patients with lymph node metastasis and those without metastasis（ P〈0. 05）. Serum BDNF levels in serous type patients were significantly higher than those in mucinous type of tumors,clear cell carcinoma and endometrial adenocarcinoma（ P〈0. 05）. However,there was no

关 键 词：子宫内膜异位症 卵巢癌 脑源性神经营养因子 

分 类 号：R711.71[医药卫生—妇产科学] R737.31[医药卫生—临床医学]