当归补血汤有效组分配伍对肝星状细胞氧化应激和Ⅱ相酶的影响  

Effects of Active Component Compatibility of Danggui Buxue Decoction on Oxidative Stress and Phase-Ⅱ Enzymes in Hepatic Stellate Cell-T6

作　　者：徐天娇[1] 张英博[1] 杨莹[2] 牛英才[1] XU Tianjiao;ZHANG Yingbo;YANG Ying;NIU Yingcai(The Institute of Medicine,Qiqihar 161006,Heilongjiang,China;The College of Pharmacy,Qiqihar Medical Univeristy,Qiqihar 161006,Heilongjiang,China)

机构地区：[1]齐齐哈尔医学院医药科学研究所,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《中华中医药学刊》2018年第9期2134-2137,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473407)

摘　　要：目的：研究当归的有效成分阿魏酸、黄芪有效成分黄芪甲苷及其配伍对大鼠肝星状细胞HSC-T6脂质过氧化物（LPO）含量、抗氧化酶及Ⅱ相解毒酶的影响。方法：阿魏酸、黄芪甲苷单独及联合处理HSC-T6细胞24 h,分光光度法检测HSC-T6细胞LPO含量和超氧化物歧化酶（（SOD）活性,Western Blot检测HSC-T6细胞谷氨酰半胱氨酸合成酶催化亚基（GCLc）、谷氨酰半胱氨酸合成酶调节亚基（GCLm）、血红素加氧酶1（HO-1）、SOD和磷酸化糖原合成酶激酶3β（p-GSK-3β）蛋白表达,实时定量PCR检测HSC-T6细胞GCLc、GCLm、HO-1和SOD1 mRNA表达。结果：黄芪甲苷显著抑制了HSC-T6细胞中LPO含量,增加了SOD活性,上调了GCLc和SOD的mRNA和蛋白表达,增加了GSK-3β的磷酸化水平（均P〈0.05）,而对GCLm表达无显著性影响（P〉0.05）。阿魏酸对这些指标均无显著性影响（均P〉0.05）。与黄芪甲苷处理组比较,联合配伍的作用效果无显著性提高（均P〉0.05）。结论：当归补血汤的有效组分黄芪甲苷通过上调Ⅱ相酶的表达抑制了肝星状细胞氧化应激。Objective： This study aims at evaluating the effect of active component compatibility of Danggui Buxue Decoction on oxidative stress and＂phase-II＂enzymes in hepatic stellate cell（ HSC）-T6. Methods： HSC-T6 cells were treated with Astragaloside Ⅳ and/or Ferulic acid for 24 h,and then cells were collected. Lipid peroxidation（ LPO） level and superoxide dismutase（ SOD） activity were determined using the spectrophotometric method. Protein expression of glutamate-cysteine ligase catalytic subunit（ GCLC）,glutamate cysteine ligase modifier subunit（ GCLm）,heme oxygenase-1（ HO-1）,SOD,and p-glycogen synthase kinase 3β（ GSK-3β） were analyzed by Western Blot. Messenger RNA expressions of GCLc,GCLm,HO-1 and SOD1 were analyzed by Real-time polymerase chain reaction（ PCR）.Results： Astragaloside Ⅳ but not Ferulic acid inhibited LPO levels,elevated SOD activity,up-regulated the protein and mRNA expressions of GCLc and SOD1,increased GSK-3β phosphorylation,while Astragaloside Ⅳ did not significantly modify GCLm expression in HSC-T6 cells. The combination treatment of Ferulic acid and Astragaloside Ⅳ failed to cause a greater effect than Astragaloside Ⅳ alone. Conclusions： Astragaloside Ⅳ,an active component of Danggui Buxue Decoction,inhibits oxidative stress through up-regulation of ＂phase-II＂enzymes expression in HSC-T6.

关 键 词：组分配伍 肝星状细胞 当归补血汤 氧化应激 Ⅱ相酶 

分 类 号：R285.5[医药卫生—中药学]