Identification of SNPs involved in regulating a novel alternative transcript of P450 CYP6ER1 in the brown planthopper  

作　　者：Zhi-Kun Liang Rui Pang Yi Dong Zhong-Xiang Sun Yan Ling Wen-Qing Zhang 

机构地区：[1]State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, China [2]Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China

出　　处：《Insect Science》2018年第5期726-738,共13页昆虫科学（英文版）

基　　金：We thank Dr. Jianming Chen （Zhejiang Academy of Agri- cultural Sciences, Hangzhou, China） for providing the susceptible population and Jiaxing field-collected pop- ulation, and Dr. Ping Wang （Cornell University, USA） for his critical comments on this manuscript. This work was funded by the National Basic Research Program of China （Grant 2010CB 126200）, National Natural ScienceFoundation of China （U 1401212） and the China Postdoc- toral Science Foundation （2016M602582）.

摘　　要：Cytochrome P450-mediated metabolic resistance is one of the major mecha- nisms involved in insecticide resistance. Although the up-regulation of cytochrome P450 plays a vital role in insecticide metabolism, the molecular basis for the transcriptional regulation of cytochrome P450 remains largely unknown. The P450 gene CYP6ERI, has been reported to confer imidacloprid resistance to the brown planthopper, Nilaparvata lugens. Here, we identified a novel alternative transcript of CYP6ER1 （transcript A2） that had different expression patterns between resistant and susceptible populations, and was more stable after insecticide induction. The promoter of this transcript was sequenced and multiple single nucleotide polymorphisms （SNPs） were detected in individuals from susceptible and resistant field-collected populations. Resistant alleles of four SNPs were found to significantly enhance the promoter activity of the CYP6ER1 transcript A2. Elec- trophoretic mobility shift assays （EMSAs） revealed that these SNPs might regulate the binding of transcription factors to the promoter. Our findings provide novel evidence re- garding the transcriptional regulation of a metabolic resistance-related gene and may be useful to understand the resistance mechanism ofN. lugens in the field.Cytochrome P450-mediated metabolic resistance is one of the major mecha- nisms involved in insecticide resistance. Although the up-regulation of cytochrome P450 plays a vital role in insecticide metabolism, the molecular basis for the transcriptional regulation of cytochrome P450 remains largely unknown. The P450 gene CYP6ERI, has been reported to confer imidacloprid resistance to the brown planthopper, Nilaparvata lugens. Here, we identified a novel alternative transcript of CYP6ER1 （transcript A2） that had different expression patterns between resistant and susceptible populations, and was more stable after insecticide induction. The promoter of this transcript was sequenced and multiple single nucleotide polymorphisms （SNPs） were detected in individuals from susceptible and resistant field-collected populations. Resistant alleles of four SNPs were found to significantly enhance the promoter activity of the CYP6ER1 transcript A2. Elec- trophoretic mobility shift assays （EMSAs） revealed that these SNPs might regulate the binding of transcription factors to the promoter. Our findings provide novel evidence re- garding the transcriptional regulation of a metabolic resistance-related gene and may be useful to understand the resistance mechanism ofN. lugens in the field.

关 键 词：alternative splicing cytochrome P450 Nilaparvata lugens single nucleotidepolymorphisms （SNPs） transcription factor binding 

分 类 号：Q343.1[生物学—遗传学] Q75