GO/β-TCP复合生物陶瓷的细胞毒性研究  

Cytotoxicity of an Innovative GO/β-TCP Hybrid Bioceramic

作　　者：卢霄 姚宸维 何佳蓉 杨艳芳 Lu Xiao;Yao Chen-wei;He Jia-rong;Yang Yan-fang(School of Materials Science and Engineering,South China University of Technology,G uangzhou 510640;Shanghai Bio-lu Biomaterials Co.,Ltd,Shanghai201114)

机构地区：[1]华南理工大学材料科学与工程学院,广东广州510640 [2]上海贝奥路生物材料有限公司,上海201114

出　　处：《生物化工》2018年第1期22-24,共3页Biological Chemical Engineering

摘　　要：将GO与β-TCP复合,通过无氧烧结工艺,形成0.1%GO复合β-TCP生物陶瓷。然后将该生物陶瓷与单相β-TCP、1%、0.1%、0.01%、0.001%GO悬浊液比较,将上述各材料的浸提液通过MTT法进行细胞毒性试验,并评价氧化石墨烯(GO)复合β-TCP生物陶瓷的细胞毒性的情况。结果表明0.1%GO复合β-TCP生物陶瓷与单相β-TCP生物陶瓷相比细胞相对增殖率无明显差异,GO悬浊液的细胞相对增殖率先随浓度降低逐渐增加,而在0.001%浓度条件下,细胞相对增殖率下降。以上各组结果均显示细胞毒性结果为0级,GO对β-TCP细胞毒性无影响。An hybrid 0.1 wt% GO/β-TCP bioceramic is obtained by sintering in inert atmosphere a slurry of GO andβ-TCP. The cytotoxicity of this material is compared with those of pure β-TCP bioceramic, 1 wt%, 0.1 wt%, 0.01 wt% and 0.001 wt% GO suspension through MTT assay, and evaluate the cytotoxicity of graphene oxide（GO） composite β-TCP bioceramics. The results show No significant difference between hybrid GO/β-TCP bioceramic and pure β-TCP bioceramic was observed on relative growth rate（RGR）. For GO suspension, a negative correlation was observed between cell proliferation and concentration in GO, but for low concentration（0.001 wt%）, a decrease in RGR was noticed. For all of the tested groups, cytotoxicity level was found to be null. The addition of GO to β-TCP ceramics had no significant impact on the cytotoxicity of the material.

关 键 词：氧化石墨烯 磷酸三钙 生物陶瓷 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]