不同方法提取肠道微生物宏基因组DNA的比较  

Comparison of different methods for extracting metagenomic DNA from intestinal microflora

作　　者：左锐 林峻[1,2] 朱琳妍 姜文倩 蔡伟文[1,2] Zuo Rui;Linjun;Zhu Lin-yan;Jiang Wen-qian;Cai Wei-wen(Institute of Applied Genomics,Fuzhou University,Fujian Fuzhou,350108;College of Biological Science and Engineering,Fuzhou University,Fujian Fuzhou 350108)

机构地区：[1]福州大学应用基因组学研究所,福建福州350108 [2]福州大学生物科学与工程学院,福建福州350108

出　　处：《生物化工》2018年第4期1-5,共5页Biological Chemical Engineering

基　　金：国家自然科学基金(31301537;31371287;31571300);福建省科技重大专项(2013YZ0002-1;2013YZ0002-2)

摘　　要：目的:比较几种不同的肠道微生物宏基因组DNA的提取方法。方法:分别使用两家不同公司的粪便基因组提取试剂盒,以及CTAB-SDS法提取肠道微生物宏基因组DNA,琼脂糖凝胶电泳、超微量核酸蛋白测定仪Nanodrop 2000、16S rDNA PCR以及二代测序等检测。结果:均能够提取出肠道微生物宏基因组DNA,PCR均能扩增出16S rDNA基因。结论:三种方法都能较好满足提取宏基因组DNA、下游分子生物学实验的要求,可根据具体实际进行选择。Objective： To compare different methods for extracting metagenomic DNA from intestinal microflora. Method： Intestinal microbial metagenomic DNA was extracted using two different commercial fecal genomic DNA extraction kits, and CTAB-SDS method. The quality of the DNA was analyzed by agarose gel electrophoresis, ultra trace nucleic acid protein analyzer Nanodrop 2000, 16S rDNA PCR and next generation sequencing. Result： The metagenomic DNA of intestinal microflora could be extracted, and 16S rDNA gene amplified by PCR. Conclusion： All three methods can meet the requirements for the extraction of metagenomic DNA and downstream molecular biology experiments, and can be selected based on actual conditions.

关 键 词：小鼠 肠道微生物 宏基因组DNA DNA提取方法 

分 类 号：Q36[生物学—遗传学]